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目的：探索保幼激素Ⅲ（ＪＨ３）对滞育三带喙库蚊卵巢滤泡和体内ＲＮＡ变化的影响。方法：短光照和低温联合作用导致三带喙库蚊滞育后，用２ｐｐｍ０．５ｕｌ或３ｐｐｍ０．５ｕｌ／蚊外源ＪＨ３点滴处理，观察卵巢滤泡发育情况，并测定蚊体内ＲＮＡ和卵巢内蛋白质含量的变化。结果：低温和短光照引起三带喙库蚊滞育后，卵巢滤泡发育受到抑制，体内ＲＮＡ的含量明显减少，ＪＨ３处理可诱导部分滞育的三带喙库蚊转入发育状态，解除滞育的个体卵巢第一滤泡增大，体内ＲＮＡ含量增加，但对卵巢蛋白的含量没有影响。结论：ＪＨ３可诱导滞育三带喙库蚊卵巢发育，体内ＲＮＡ合成增加。
Objective: To explore the effect of juvenile hormone Ⅲ (JH3) on the changes of ovarian follicles and the changes of RNA in Culex. Methods: Short-term exposure to light and low temperature resulted in the diapause of Culex tritaeniorhynchus. After treatment with 2ppm 0.5ul or 3ppm 0.5ul / mosquito exogenous JH3, the development of ovarian follicles was observed, and the contents of RNA and ovary protein Changes in content. Results: The development of ovarian follicles was inhibited after cold and light exposure, and the content of RNA in body was significantly reduced. JH3 treatment induced partial diapause of Culex tritaeniorhynchus into developing state, Fertility of the ovarian follicles increased the first increase in body RNA content, but the ovarian protein content has no effect. Conclusion: JH3 can induce oviparous development of Culex tritaeniorhynchus in diapause, and increase the synthesis of RNA in vivo.