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目的探讨细胞色素CYP1A1基因MSPI多态性与膀胱癌遗传易感性的关系。方法应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析技术,检测44例膀胱癌患者(病例组)和85例同期住院非膀胱癌患者(对照组),检测CYP1A1基因MSPI多态性位点的3种基因型及等位基因的分布频率。结果在病例组CYP1A1基因MSPI位点基因型分布频率为:TT(54·5%)、TC(36·4%)、CC(9·1%),等位基因分布频率为T(72·7%)、C(27·3%);在对照组CYP1A1基因MSPI位点基因型的分布频率为TT(61·2%)、TC(31·2%)、CC(7·1%),等位基因分布频率为T(77·1%)、C(22·9%)。各个基因型在两组中所占的比例差异无统计学意义(P>0·05);T、C等位基因频率两组比较差异亦无统计学意义(P>0·05)。结论CYP1A1基因MSPI位点多态性的单独存在可能与本地区膀胱癌易感性无关。
Objective To investigate the relationship between the MSPI polymorphism of cytochrome CYP1A1 gene and genetic susceptibility to bladder cancer. Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to detect the expression of CYP1A1 gene in 44 patients with bladder cancer (case group) and 85 patients with non-bladder cancer in the same period (control group) Three genotypes of MSPI polymorphism loci and distribution frequency of alleles. Results The distribution frequency of CYP1A1 gene MSPI locus was TT (54.5%), TC (36.4%), CC (9.1%), allele frequency was T (72.7% (27.2%), TC (31.2%), CC (7.1%), and C (27.3%) respectively. The frequency of CYP1A1 gene MSPI locus in control group was 61.2% The distribution frequency of the gene was T (77.1%) and C (22.9%). There was no significant difference in the proportions of genotypes in the two groups (P> 0.05). There was no significant difference in the frequencies of T and C alleles between the two groups (P> 0.05). Conclusion The existence of MSPI polymorphism of CYP1A1 gene alone may not be related to the susceptibility to bladder cancer in this region.