论文部分内容阅读
目的探讨化学发光微粒子酶免疫分析法定量检测开封市区从业人员抗HAV-IgG含量的临床应用价值。方法分别采用化学发光微粒子免疫法和ELISA法对664份血清进行抗HAV-IgG定性检测,对598份抗HAV-IgG阳性样本使用化学发光微粒子免疫法定量检测。结果化学发光微粒子酶免疫分析法阳性率为90.06%,ELISA法阳性率为83.73%,化学发光微粒子酶免疫分析法阳性率高于ELISA法,差异有统计学意义(P<0.01)。从业人员抗HAVIgG S/CO值随着年龄的增长逐渐升高,女性的S/CO值高于男性。结论相较于传统的ELISA法,化学发光微粒子免疫检测法检测阳性率更高,结果更精确,值得临床推广应用。
Objective To investigate the clinical value of chemiluminescence microparticle enzyme immunoassay in the quantitative determination of anti-HAV-IgG in Kaifeng practitioners. Methods The chemiluminescence microparticle immunoassay and ELISA were used to detect the anti-HAV-IgG in 664 serum samples, and 598 anti-HAV-IgG positive samples were detected by chemiluminescence microparticle immunoassay. Results The positive rate of chemiluminescence microparticle enzyme immunoassay was 90.06%. The positive rate of ELISA was 83.73%. The positive rate of chemiluminescence microparticle enzyme immunoassay was higher than that of ELISA. The difference was statistically significant (P <0.01). Employees anti-HAVIgG S / CO values gradually increased with age, women’s S / CO value higher than men. Conclusion Compared with the traditional ELISA method, chemiluminescence microparticle immunoassay has a higher positive rate and more accurate results, which is worthy of clinical application.