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目的探讨脑内定向移植神经干细胞(NSCs)治疗脑出血的可行性和机制。方法采用在成年大鼠中立体定向纹状体内注入新鲜自体血液制作实验性脑出血模型,术后1d采用立体定向将在体外扩增、纯化并经BrdU标记的神经干细胞移植入脑出血部位,通过神经功能缺损评分来观察移植后大鼠神经行为学改善情况,并通过双标GFAP、NeuN、BrdU免疫组化来检测移植入脑的NSCs。结果移植的NSCs迁移至血肿周围区,并分化为神经元(≈10%BrdU阳性细胞)和星形胶质细胞(≈75%);与对照组比较,NSCs移植组在移植后1周有更好的神经功能缺损评分,而且此疗效可维持长达8周。结论移植的NSCs可在脑出血的大鼠脑中存活、迁移,并促进功能恢复。立体定向移植神经干细胞可改善实验性脑出血大鼠的神经功能。
Objective To investigate the feasibility and mechanism of intracerebral transplantation of neural stem cells (NSCs) for cerebral hemorrhage. Methods Experimental cerebral hemorrhage model was established by injecting fresh autologous blood into the stereotactic striatum of adult rats. After 1 d, the neural stem cells were expanded, purified and labeled with BrdU in vitro. Neurological deficit score was used to observe the neurobehavioral improvement of transplanted rats. NSCs transplanted into brain were detected by double labeled GFAP, NeuN, BrdU immunohistochemistry. Results The transplanted NSCs migrated to the perihematomal area and differentiated into neurons (≈10% BrdU positive cells) and astrocytes (≈75%). Compared with the control group, the NSCs transplantation group had more changes at 1 week after transplantation Good neurological deficit score, and this effect can be maintained for up to 8 weeks. Conclusion Transplanted NSCs can survive, migrate and promote functional recovery in ICH rats. Stereotactic transplantation of neural stem cells can improve the neurological function of experimental intracerebral hemorrhage in rats.