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目的基于实时细胞分析技术结合HPLC法,比较不同部位、不同产地雷公藤的生物活性。方法建立x CELLigence实时细胞分析系统实时动态检测细胞活性的方法:接种密度为2×105/m L,给药时间为24 h左右,检测雷公藤对RBL-2H3细胞的活性;通过HPLC法建立不同部位雷公藤的指纹图谱并分析其共有峰。结果浙江产雷公藤同一部位活性相似;根皮和叶具有类似生物学活性,根和茎具有类似的活性,根皮、嫩芽对RBL-2H3细胞的生物活性明显高于根、茎部位,尤以根皮部活性最强;指纹图谱显示不同部位成分相似但量差异较大;氯仿提取液对RBL-2H3细胞的抑制率明显高于水提液。结论在细胞生物学层面上雷公藤根皮及地上部分有研究与药用价值,可对其深入开发和利用。
OBJECTIVE: To compare the bioactivity of Tripterygium wilfordii in different parts and areas based on real-time cell analysis and HPLC. Methods Real-time dynamic detection of cell viability by real-time xCELLigence real-time cell analysis system was established. The inoculation density was 2 × 105 / m L and the administration time was about 24 h. The activity of Tripterygium wilfordii against RBL-2H3 cells was determined. Part Tripterygium fingerprinting and analysis of its common peak. The results showed that the same part of Tripterygium wilfordii produced in Zhejiang was similar in activity. The root bark and leaves had similar biological activity and similar activities in roots and stems. The biological activities of root bark and shoots in RBL-2H3 cells were significantly higher than those in roots and stems. The strongest activity was found in the root bark. The fingerprints of the RBL-2H3 cells were similar to those of the water extract. Conclusion In the cell biology level, the root bark and aerial parts of Tripterygium wilfordii have research and medicinal value, which can be further developed and utilized.