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目的 :探讨大肠癌组织细胞的增殖活性与病理学特点及生存率的关系。方法 :应用兔抗人 Ki- 6 7多克隆抗体 ,对 10 7例大肠癌术后标本进行 S- P免疫组化检测。结果 :大肠癌组织的 Ki- 6 7标记指数明显高于正常肠粘膜及癌旁粘膜 (P <0 .0 1) ;伴淋巴结转移和远隔脏器转移之大肠癌组织的 Ki- 6 7标记指数明显高于无转移者 (P <0 .0 5 ) ;大肠癌 Ki- 6 7表达阳性强的病人 ,术后生存率低于 Ki- 6 7表达弱者 (P<0 .0 5 ) ;Ki- 6 7的表达与肿瘤大小、生长方式、浸润深度及组织分化程度无明显相关 (P >0 .0 5 )。结论 :Ki- 6 7作为代表肿瘤增殖活性的标志 ,与大肠癌的转移和预后密切相关
Objective: To investigate the relationship between the proliferation activity, pathological features and survival rate of colorectal cancer tissue cells. METHODS: S-P immunohistochemistry was performed on 107 specimens of colorectal cancer using rabbit anti-human Ki-67 polyclonal antibody. Results: The Ki-67 labeling index of colorectal cancer tissue was significantly higher than that of normal intestinal mucosa and paraneoplastic mucosa (P < 0.01); Ki-67 marker of colorectal cancer tissues with lymph node metastasis and distant organ metastasis. The index was significantly higher than those without metastasis (P <0.05). The patients with strong expression of Ki-67 in colorectal cancer had lower postoperative survival rates than those with weak Ki-67 expression (P <0.05). - The expression of 67 was not significantly associated with tumor size, growth pattern, depth of invasion, and tissue differentiation (P > 0.05). Conclusion: Ki-67 as a marker of tumor proliferation activity is closely related to the metastasis and prognosis of colorectal cancer.