论文部分内容阅读
目的:建立大鼠头部暴露吸入染毒致急性肺损伤实验模型以便进行全氟异丁烯 (perfluoroisobutylene,PFIB)毒理及防治药物研究。方法:用设计的大鼠头部暴露吸入PFIB染毒装置致动物中毒,测定动物中毒后肺灌流液(BALF)中总蛋白含量和肺湿干比变化,在染毒过程中同时用气相色谱连续监测PFIB浓度。结果:在大鼠头部暴露吸入染毒中,PFIB染毒浓度比较稳定。大鼠吸入PFIB0. 18mg/L(8~10min)致肺损伤实验模型比较明显,各项指标变化显著,中毒性肺水肿程度与PFIB浓时积 (染毒浓度乘以时间,即CT值 )呈正相关,动物中毒后 12 ~24h肺灌流液中总蛋白含量和肺湿干比显著升高。结论:建立了大鼠头部暴露动态吸入PFIB中毒致急性肺损伤实验模型,该模型比较稳定,可用于观察动物PFIB中毒性肺水肿程度,也可作为PFIB毒理及防治药物研究实验方法。大鼠PFIB中毒途径主要通过呼吸道吸入中毒,其吸入毒性至少比腹腔毒性大 10倍。
OBJECTIVE: To establish an experimental model of acute lung injury induced by exposure to inhalation in rats for the study on toxicology and drug control of perfluoroisobutylene (PFIB). Methods: The animals exposed to PFIB exposure device were exposed to the designed head of rats. The total protein content and lung wet-dry ratio in the lung perfusion fluid (BALF) were determined after the animal was poisoned. Meanwhile, Monitor PFIB concentration. Results: Exposure to exposure to the head of rats in the PFIB exposure concentration was relatively stable. Rat model of lung injury induced by inhalation of PFIB0.18mg / L (8 ~ 10min) was obvious, and the indexes changed significantly. The degree of toxic pulmonary edema was positively correlated with the concentration of PFIB concentrated time multiplied by time, namely CT value Related, 12 ~ 24h animal poisoning after perfusion of total protein and lung wet-dry ratio was significantly higher. CONCLUSION: An experimental model of acute lung injury induced by dynamic inhalation of PFIB in rat head is established. The model is stable and can be used to observe the degree of pulmonary edema caused by PFIB in animals. It can also be used as experimental method for toxicological and preventive drug treatment of PFIB. The pathways of PFIB poisoning in rats are mainly inhaled through the respiratory tract, and their inhalation toxicity is at least 10 times greater than that of abdominal cavity.