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(一)材料和方法 1.菌种:青霉(Penicillium chrysogenum),黑曲霉(Aspergillus niger) 2.培养:将青霉和黑曲霉斜面制成悬液,吸0.1ml于马铃薯培养基(马铃薯200克,蔗糖20克,琼脂粉11克,水1000毫升,15磅/吋~2灭菌30分钟)平板上涂布,于28℃培养2天。 3.制片:取两块干净载玻片,在其中一块上涂一层薄胶层(胶为光学树脂),然后用解剖针在青霉或曲霉平板上挖一小块带菌琼脂(约0.5厘米~2),将琼脂块放在薄胶层上(培养面朝下),用另一块载玻片在琼脂块上轻轻按压,然后将琼脂块小心弃去。将印有菌迹的载玻片
(A) Materials and methods 1. Strain: Penicillium chrysogenum, Aspergillus niger 2. Culture: A suspension of Penicillium and Aspergillus niger was slurried and aspirated 0.1 ml in a potato medium (Potato 200 G, sucrose 20 g, agar powder 11 g, water 1000 ml, sterilized at 15 lbs / in ~ 2 for 30 minutes) and plated at 28 ° C for 2 days. 3. Preparation: Take two clean glass slides and apply a thin layer of adhesive (plastic for the optical resin) on one of the pieces. Then use a dissection needle to dig a small piece of fungal agar (about 0.5 Cm ~ 2), place the agar block in a thin layer (with the culture side down), gently press on the agar block with another slide, and discard the agar block carefully. Bacterial slides will be printed