目的:建立薄层色谱法(TLC)和高效液相色谱法(HPLC),对紫锥菊不同部位中的菊苣酸进行分析,为紫锥菊质量标准的建立提供依据。方法:TLC鉴别紫锥菊根、茎、叶、花中的菊苣酸;HPLC测定以上各部位中菊苣酸的含量,色谱柱为XTerraC18柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(23︰77),流速为1.0 mL.min-1,紫外检测波长为326 nm。结果:TLC中菊苣酸的斑点清晰可辨,Rf值为0.72;HPLC测得菊苣酸的进样浓度在0.5～100μg.mL-1范围内与峰面积呈良好的线性关系(r=0.9999),方法的平均回收率(n=3)为95.3%(RSD=3.6%),紫锥菊根、茎、叶、花中的菊苣酸含量分别为12.1,0.68,5.60,2.47 mg.g-1。结论:该检测方法简便可行,可用于紫锥菊中菊苣酸的质量控制。 OBJECTIVE: To establish a TLC and HPLC method for the analysis of ciclofenac in different parts of Echinacea purpurea and provide basis for the establishment of Echinacea quality standard. METHODS: The contents of cichorine in roots, stems, leaves and flowers of Echinacea purpurea were determined by TLC. The contents of cichorine in the above fractions were determined by HPLC. The column was XTerraC18 column (150 mm × 4.6 mm, 5 μm) with the mobile phase of acetonitrile 0.1% Phosphoric acid solution (23:77) was used at a flow rate of 1.0 mL.min-1. The UV detection wavelength was 326 nm. Results: The spot of chicory acid in TLC was clear and the Rf value was 0.72. The linearity (r = 0.9999) for the concentration of chicory acid in the range of 0.5-100μg.mL-1 was found by HPLC. The average recoveries (n = 3) were 95.3% (RSD = 3.6%). The contents of chicory acid in root, stem, leaf and flower of Echinacea purpurea were 12.1,0.68,5.60 and 2.47 mg.g-1, respectively. Conclusion: The method is simple and feasible and can be used for quality control of chicory acid in Echinacea purpurea.