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肠道微生物与宿主的健康状况息息相关,已成为当今的热点研究领域。随着分子生物学技术的快速发展,高通量测序技术、实时荧光定量PCR技术和PCR-DGGE技术等凭借其高灵敏度、高通量、无需体外培养等优势,为研究微生物结构和功能基因组提供了新方法,并在肠道菌群的研究中应用广泛。本文对这三种分子生物学技术在肠道微生物研究中的应用进行了综述,总结了这三种技术在肠道微生物研究中的应用范围和优缺点,并展望了其在肠道微生物研究中的广阔应用前景。
Gut microbiota is closely related to the health status of the host and has become a hot research field today. With the rapid development of molecular biology technology, high-throughput sequencing, real-time fluorescence quantitative PCR and PCR-DGGE technology, with their advantages of high sensitivity, high throughput and no need of in vitro culture, provide the scientific basis for the study of microbial structure and functional genomics The new method, and in the study of intestinal flora widely used. In this paper, the applications of these three molecular biological techniques in gut microbiology research are reviewed. The application scope, advantages and disadvantages of these three techniques in gut microbiology research are summarized, and their applications in gut microbiology The broad application prospects.