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采用 HPL C法 ,以 Shim-pack CL C-ODS为色谱柱 ,乙腈 -0 .0 2 mol· L- 1 磷酸二氢钠 (60∶ 40 )为流动相 ,检测波长 2 1 0 nm,测定复方狼毒注射液中苦参碱的含量。结果 :苦参碱在 0 .1 5～ 0 .90 mg· ml- 1范围内呈线性关系 ,γ=0 .9993 ,平均回收率为 99.1 3 % ,RSD=0 .99%。方法简便快速 ,结果准确可靠 ,可作为该制剂的质量控制方法。
The HPL C method was used on a Shim-pack CL C-ODS column with acetonitrile-0.02 mol·L-1 monobasic sodium phosphate (60:40) as the mobile phase and the detection wavelength at 210 nm. The content of matrine in Wolfoo injection. RESULTS: The matrine ranged from 0.15 to 0.090 mg·ml-1, with a linear relationship of γ=0.9993. The average recovery was 99.1 3% with RSD=0.99%. The method is simple and rapid, and the result is accurate and reliable. It can be used as a quality control method for the preparation.