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目的 CD+ 4T细胞通过间接识别途径识别MHC Ⅰ类移植抗原而活化已在离体实验中得到证实 ,但体内实验尚未见报道。方法 bml小鼠 (H 2Kbm1∶Kbm1,Db,Ⅰ Ab)的皮肤片分别移植到①B6小鼠 (H 2 b∶Kb,Db,Ⅰ Ab,n =8) ;②B6CD / 8小鼠 (H 2 b∶Kb,Db,Ⅰ Ab,CD / 8,n =8) ;③用抗CD4 抗体处理的B6CD / 8小鼠体上 (n =8)。观察移植皮肤的生长时间 ;用流式细胞仪 (FACScan)观察各组小鼠内T细胞亚群的变化。各组数据用分组或配对t检验进行数据统计。结果 尽管CD / 8小鼠体内未发现CD+ 8T细胞存在 ,但很快排斥了bm1皮肤移植片 (2 1 9d± 1 9d) ;而用抗CD4 抗体处理的B6CD / 8小鼠却长时间接受bm1皮肤移植片 (>47 5d± 3 8d) ;用流式细胞仪发现CD+ 4T细胞明显增殖活化并被抗CD4 抗体抑制。结论 CD+ 4T细胞通过间接识别途径活化并导致同种相异MHC Ⅰ类移植物的排斥。
Objective CD + 4T cells were identified by indirect identification of MHC class Ⅰ transplantation antigen activation has been confirmed in vitro experiments, but in vivo experiments have not been reported. Methods B7 mice (H 2 b: Kb, Db, Ⅰ Ab, n = 8) were transplanted into the skin of bml mice (H 2Kbm1: Kbm1, Db and Ⅰ Ab) : Kb, Db, I Ab, CD / 8, n = 8); ③ on B6CD / 8 mice treated with anti-CD4 antibody (n = 8). The growth of transplanted skin was observed. The changes of T cell subsets in each group were observed by flow cytometry (FACScan). Data for each group were grouped or paired t-test for data statistics. Results Although no CD + 8 T cells were found in CD / 8 mice, bm1 skin grafts were rapidly rejected (219d ± 19d), whereas B6CD / 8 mice treated with anti-CD4 antibody received bm1 for a long time Skin graft (> 47d ± 38d). CD + 4T cells were found to proliferate and be inhibited by anti-CD4 antibody by flow cytometry. Conclusion CD + 4T cells are activated by indirect recognition and lead to the rejection of MHC class Ⅰ allografts.