论文部分内容阅读
目的探讨溃疡性结肠炎患者结肠黏膜β防御素-2(HBD-2)的表达及临床意义。方法 2009年5月—2012年12月本院收治的溃疡性结肠炎患者30例为溃疡性结肠炎组,肠易激综合征患者30例为肠易激综合征组,另选择健康体检者30例为对照组。3组患者入院均行电子结肠镜检查,收集到的活检组织一部分立即提取RNA用于PT-PCR检测,另一部分进行HBD-2免疫组织化学染色(SP法)。比较各组结肠黏膜HBD-2 mRNA表达水平、结肠黏膜中HBD-2表达阳性率、结肠黏膜中HBD-2表达强度。结果溃疡性结肠炎组、肠易激综合征组患者结肠黏膜HBD-2 mRNA水平均明显高于对照组。溃疡性结肠炎组患者结肠黏膜HBD-2 mRNA水平明显高于肠易激综合征组。溃疡性结肠炎组结肠黏膜HBD-2阳性表达率明显高于肠易激综合征组、对照组。溃疡性结肠炎组结肠黏膜中HBD-2表达强度(2+、3+)明显强于肠易激综合征组、对照组。结论 HBD-2在溃疡性结肠炎的发生发展中发挥重要作用。
Objective To investigate the expression of colonic mucosal β-defensin-2 (HBD-2) in patients with ulcerative colitis and its clinical significance. Methods From May 2009 to December 2012, 30 patients with ulcerative colitis admitted to our hospital were selected as ulcerative colitis group, 30 patients with irritable bowel syndrome as irritable bowel syndrome group, and 30 healthy volunteers Example for the control group. All patients in the three groups underwent electronic colonoscopy. One part of the collected biopsies were immediately extracted for PT-PCR and the other for HBD-2 immunohistochemistry (SP method). The expression of HBD-2 mRNA, the positive rate of HBD-2 in colonic mucosa and the expression intensity of HBD-2 in colonic mucosa were compared between the groups. Results The levels of HBD-2 mRNA in colonic mucosa of patients with ulcerative colitis and irritable bowel syndrome were significantly higher than those of the control group. The level of HBD-2 mRNA in colonic mucosa of patients with ulcerative colitis was significantly higher than that of irritable bowel syndrome. The positive expression rate of HBD-2 in colonic mucosa of ulcerative colitis group was significantly higher than that of irritable bowel syndrome group and control group. The expression of HBD-2 in colonic mucosa (2 +, 3 +) in ulcerative colitis group was significantly stronger than that in irritable bowel syndrome group and control group. Conclusion HBD-2 plays an important role in the occurrence and development of ulcerative colitis.