论文部分内容阅读
目的:观察刺梨提取物CL1对胃癌SGC-7901细胞增殖和人脐血CD34+造血干/祖细胞(HSPC)增殖分化能力的影响。方法:采用MTT法测定活细胞OD值,计算CL1对胃癌SGC-7901细胞增殖抑制率。免疫磁珠分选人脐血CD34+造血干/祖细胞,细胞记数、双色直接免疫荧光标记法和流式细胞仪分析细胞增殖分化能力变化。结果:MTT检测法显示,0.01~10μmol.L-1的CL1处理,剂量依赖性和时间依赖性地抑制胃癌SGC-7901细胞生长。经14 d的培养,与细胞对照组相比,10、1μmol.L-1的CL1细胞数有下降趋势,但差异无显著性;表达粒系/单核巨噬系细胞表型CD15和CD11b的细胞百分数无显著差异。经14 d的培养,与培养0 d比较,CD15显著增高(P<0.05),CD11b有增高趋势,但无显著性差异。结论:CL1对胃癌SGC-7901细胞的体外生长具有抑制作用,但对人脐带血CD34+HSPC的增殖、和向粒细胞、单核巨噬细胞分化无明显影响,表明CL1在抗肿瘤作用剂量下,不会明显抑制造血干/祖细胞向粒-单细胞的增殖分化,提示CL1可能不会引起明显的造血抑制。
OBJECTIVE: To observe the effect of Cili Pullorum Extract CL1 on proliferation and differentiation of gastric cancer SGC-7901 cells and human cord blood CD34 + hematopoietic stem / progenitor cells (HSPCs). Methods: The OD value of live cells was determined by MTT method, and the inhibitory rate of CL1 on SGC-7901 cells proliferation was calculated. Immunomagnetic separation of human umbilical cord blood CD34 + hematopoietic stem / progenitor cells, immunocytochemistry, two-color direct immunofluorescence labeling and flow cytometry analysis of cell proliferation and differentiation. Results: MTT assay showed that the CL1 treatment of 0.01 ~ 10μmol.L-1 inhibited the growth of gastric cancer SGC-7901 cells in a dose-dependent and time-dependent manner. After 14 days of culture, the number of CL1 cells in 10,1 μmol.L-1 cells decreased compared with that in the cell control group, but the difference was insignificant. The expression of CD15 and CD11b in the granulocyte / monocyte-macrophage cell line No significant difference in cell percentage. After 14 days of culture, CD15 was significantly increased (P <0.05) and CD11b was increased, but no significant difference was found. CONCLUSION: CL1 can inhibit the growth of gastric cancer SGC-7901 cells in vitro, but it has no obvious effect on the proliferation of human cord blood CD34 + HSPCs and the differentiation into granulocytes and monocyte-macrophage cells, indicating that CL1 has an antitumor effect , Will not significantly inhibit hematopoietic stem / progenitor cells to granulosa - single cell proliferation and differentiation, suggesting that CL1 may not cause significant hematopoietic suppression.