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目的:建立根痛平胶囊中芍药苷的含量测定方法。方法:色谱柱:Inertsil ODS-SP(4.6×250mm,5μm),流动相甲醇:0.1%醋酸(15:85),流速1.0m L·min-1,检测波长:230nm,柱温:30℃。结果:芍药苷在2.02~16.18μg·m L-1呈良好的线性关系y=7543x-14312(r=09998),平均回收率为100.21%,RSD=1.58%结论:本法灵敏、准确、专属性强,重现性好,可作为根痛平胶囊中芍药苷的有效测定方法 。
Objective: To establish a method for the determination of paeoniflorin in root pain capsule. Methods: Column: Inertsil ODS-SP (4.6 × 250 mm, 5 μm), mobile phase methanol: 0.1% acetic acid (15:85), flow rate 1.0 mL · min-1, detection wavelength 230 nm, column temperature 30 ℃. Results: Paeoniflorin showed a good linear relationship with 2.02-16.18 μg · mL -1 y = 7543x-14312 (r = 09998). The average recovery was 100.21% and RSD was 1.58%. Conclusion: The method is sensitive, accurate and specific Strong, reproducible, and can be used as an effective method for determination of paeoniflorin in root pain capsule.