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目的 :探讨油酸肺损伤所致 RDS过程进行性氧分压下降与肝组织脂质过氧化、中分子物质和一氧化氮、内皮素以及肝功能动态变化之间的关系。方法 :随机取日本大耳白兔 36只 ,分为对照组和油酸 30 min、6 0 m in、90 m in、12 0 min组。检测 MABP、血气以及血浆和肝组织 GSH - Px、MDA、MMS、NO、ET和肝功能的动态变化 ,同时进行肝组织电镜观察。结果 :实验组 Pa O2 和 MABP随时间延长呈进行性下降。反映肝功能的血浆 AL T、AST、AL P含量在实验组分别从 6 0 m in、12 0 m in和 30 m in后逐渐增加。血浆 AL P和 AL T含量与 Pa O2 变化之间呈负相关关系 ;与肝组织 MDA含量变化之间呈正相关关系 ;与肝组织 MMS含量变化之间也呈正相关关系。实验组血浆和肝组织 GSH- Px含量随时间延长逐渐降低 ,而 MDA含量却随时间延长逐渐增加。肝组织 GSH - Px和 MDA含量变化与 Pa O2 下降之间分别呈正相关和负相关关系。肝组织 GSH- Px与 MDA含量变化之间呈负相关关系 ;肝组织NOS含量在油酸注入后 30 min时代偿性增加 ,而从 6 0 min后又逐渐降低。实验组血浆和肝组织 MMS含量随时间延长逐渐增高。肝组织 MMS含量与 Pa O2 变化之间呈负相关关系。结论 :油酸所致单纯肺损伤过程中可伴发肝功能和肝结构的损伤 ,此损伤变化与 Pa
OBJECTIVE: To investigate the relationship between the progressive partial pressure of oxygen (PO2) and lipid peroxidation, the changes of middle molecular substances, nitric oxide, endothelin and hepatic function in RDS induced by oleic acid-induced lung injury. Methods: Thirty-six Japanese white rabbits were randomly divided into control group and oleic acid for 30 min, 60 min, 90 min, 120 min. The dynamic changes of MABP, blood gas, GSH - Px, MDA, MMS, NO, ET and liver function in plasma and liver tissue were detected. Liver tissue was also observed under electron microscope. Results: Pa O2 and MABP in the experimental group decreased progressively with time. The levels of ALT, AST and ALP in plasma reflecting the liver function increased gradually after 60 minutes, 120 minutes and 30 minutes respectively in the experimental group. There was a negative correlation between plasma ALP and ALT contents and the changes of PaO 2, a positive correlation with changes of MDA content in liver tissues, and a positive correlation with changes of MMS contents in liver tissues. The content of GSH-Px in plasma and liver tissue of experimental group decreased gradually with time, but the content of MDA gradually increased with time. There was a positive correlation and a negative correlation between the change of GSH - Px and MDA content and the decrease of Pa O2 in liver tissue. There was a negative correlation between the content of GSH-Px and the content of MDA in liver tissue. The content of NOS in liver tissue was compensated at 30 min after oleic acid injection, but decreased from 60 min later. The MMS content of plasma and liver tissue in experimental group increased gradually with time. There was a negative correlation between the content of MMS and the change of Pa O2 in liver tissue. Conclusion: Oleic acid-induced lung injury can be accompanied by liver function and liver damage during injury. The changes of injury and Pa