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为了探索常温下（２２℃）保存离体肝脏的可能性，本实验以ＨＴＫ液为基础，氟碳乳剂为氧载体，脂肪乳剂为能量，常温下充氧持续灌注离体猪肝门静脉。定时取灌注液测量肝生化指标，观察肝细胞线粒体大小及密度。发现含氧载体灌注液中氨浓度下降，尿素浓度升高，线粒体损伤较轻；加入脂肪乳剂则能维持葡萄糖的浓度。各项指标与对照组比较差异有显著意义。说明利用氧载体（氟碳乳剂）为离体肝提供足够的氧，同时给予足够的能量（脂肪乳剂）时，离体肝脏就能在较高温度下较长时间（４０小时）地维持它的主要解毒功能——摄取氨合成尿素，同时亦可维持肝细胞线粒体的稳定性。可见常温下保存离体肝脏是可能的。
In order to explore the possibility of preserving ex vivo liver at room temperature (22 ℃), this experiment was based on HTK fluid, fluorocarbon emulsion as oxygen carrier and fat emulsion as energy. The perfusion fluid was taken regularly to measure the liver biochemical indexes, and the mitochondrial size and density of hepatocytes were observed. It was found that the concentration of ammonia in the perfusate of oxygenated vehicle decreased, the concentration of urea increased, and mitochondria were less damaged. The addition of fat emulsion could maintain the concentration of glucose. The indicators compared with the control group have significant differences. This shows that when oxygen carrier (fluorocarbon emulsion) is used to provide sufficient energy to the ex-liver, sufficient exergy (fat emulsion) is administered to the ex-liver to maintain it at a relatively high temperature for a longer period of time (40 hours) The main detoxification function - uptake of ammonia synthesis of urea, but also to maintain the stability of liver cells mitochondria. It is possible to store isolated liver at room temperature.