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对卵巢癌COC1细胞进行体外培养,当培养至109/mL浓度时裂解细胞,获得细胞总蛋白,利用二维电泳对顺铂诱导前后的细胞内总蛋白质进行分离,利用PDQuest 7.1.1专业分析软件对二维凝胶图谱进行差异比较,利用基质辅助激光解析飞行时间质谱(MALDI-TOF-MS)进行质量分析,最后进行生物信息学检索,以寻找卵巢癌细胞发生、发展和凋亡的分子标记物.经二维电泳图谱和质谱分析,鉴定了11种蛋白,4种蛋白表达量有差异,7种蛋白在诱导前后表现为有和无的关系.有5种是“分子伴侣”蛋白,有4种蛋白与人体能量代谢有关.蛋白功能分析提示卵巢癌细胞的蛋白质组表达存在差异,这些差异蛋白对于寻找卵巢癌生物标志物,开发新药提供很好的理论依据.
The ovarian cancer COC1 cells were cultured in vitro. When cultured to a concentration of 109 / mL, the cells were lysed to obtain the total cellular protein. The two-dimensional electrophoresis was used to separate the total intracellular proteins before and after cisplatin induction. PDQuest 7.1.1 professional analysis software The differences of two-dimensional gel were compared, and mass spectrometry was performed by MALDI-TOF-MS. Finally, bioinformatics search was performed to find the molecular markers of ovarian cancer cell development, development and apoptosis The results of two-dimensional gel electrophoresis and mass spectrometry showed that 11 proteins were identified and the expression levels of the four proteins were different, and the seven proteins showed a relationship with and without induction before and after induction. Five of them were “chaperones” , There are four proteins related to the energy metabolism of human body.Analysis of protein function suggests that proteomic expression of ovarian cancer cells are different, these differential proteins provide a good theoretical basis for finding ovarian cancer biomarkers and developing new drugs.