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目的:探讨旋转磁场对放射损伤小鼠肺损伤的影响。方法:132只雄性BALB/c小鼠随机分为正常组(N),单纯磁疗组(M),单纯照射组(R)和照射磁疗组(R+M)4组,R组和R+M组小鼠接受吸收剂量6.0Gy的60Coγ射线全身一次照射,M组及N组不予以照射,M组及R+M组予以磁场处理30d,每天2次,每次l.5h,分别于第9,23、30d测定小鼠湿肺羟脯氨酸含量,并进行小鼠肺光镜、电镜超微结构的观察。结果:N组和M组肺羟脯氨酸含量在3个时间点上都低于R组和R+M组,有明显差别(P均<0.05)。R组和R+M组肺羟脯氨酸含量在3个时间点均无明显差别(P均<0.05)。苏木精-伊红染色结果显示,N组肺组织结构正常;照射后d9,R组及R+M组肺泡壁轻度增厚,普遍肺毛细血管扩张、充血,少量淋巴细胞、中性粒细胞浸润。小鼠肺组织透射电镜观察结果显示,照射后d30,N组及M组肺间质胶原纤维正常无增生;R组及R+M组肺可见肺间质胶原纤维较N组及M组明显增多,但R+M组与R组比较差别不明显。结论:6.0Gy的60Coγ射线照射后小鼠的肺受到了损伤,但磁场没有加重这种损伤且磁疗对正常小鼠无明显肺损伤。
Objective: To investigate the effect of rotating magnetic field on lung injury in radiation-injured mice. Methods: Thirty-two male BALB / c mice were randomly divided into 4 groups: normal group (N), magnetic therapy alone group (R) and irradiation magnetic therapy group (R + M) + M mice received whole body irradiation of 60 Gy with an absorbed dose of 6.0 Gy, M group and N group were not irradiated, and M group and R + M group were subjected to magnetic field treatment for 30 days twice a day for 1.5 hours respectively On the 9th, 23th, 30th and 30th days, the content of hydroxyproline in wet lung of mice was measured, and the ultrastructure of the lungs was observed by light microscopy and electron microscopy. Results: The contents of hydroxyproline in N and M groups were significantly lower than those in R group and R + M group at 3 time points (P <0.05). The contents of hydroxyproline in R group and R + M group were not significantly different at 3 time points (all P <0.05). The results of hematoxylin-eosin staining showed that the structure of lung tissue in group N was normal. The alveolar walls in group R9 and group R were slightly thickened after irradiation, and pulmonary telangiectasia, congestion, few lymphocytes and neutrophils Cell infiltration. Transmission electron microscopy results of lung tissue in mice showed that the normal group had no proliferation of collagen fibers in group D and group N at the 30th day after irradiation. The number of pulmonary interstitial collagen fibers in group R and M + , But the difference between R + M group and R group was not obvious. CONCLUSIONS: The lungs of mice were damaged after 6.0Gy of 60Coγ-ray irradiation, but the magnetic field did not aggravate the damage and the magnetic therapy had no obvious lung injury to normal mice.