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目的：研究丝裂原素在人单核细胞白细胞介素２（ＩＬ－２）受体的表达及杀伤机制中的作用。方法：采用多种丝裂原素处理人单核细胞，观察其对单核细胞ＩＬ－２受体α链表达和杀伤活性的影响。结果：美洲商陆（ＰＷＭ）和脂多糖（ＬＰＳ）在处理２４小时后诱导人单核细胞表达ＩＬ－２受体α链；植物血凝素（ＰＨＡ）只有在处理４８小时后才观察到有诱导α链表达作用；而刀豆素Ａ（ＣｏｎＡ）无明显诱导作用。在以上丝裂原素预处理后，加入ＩＬ－２，在有诱导ＩＬ－２受体α链表达的处理组，可见其单核细胞杀伤肿瘤细胞（Ｕ９３７）的活性明显提高。其中以ＰＷＭ预处理组的单核细胞活性增加最为显著。用流式细胞仪检测到ＰＷＭ可同时与单核细胞和Ｕ９３７靶细胞结合。经ＰＷＭ预处理后，加入ＩＬ－２，明显见到Ｕ９３７细胞发生凋亡的现象（包括核浓缩、凋亡小体的形成和ＤＮＡ的断裂）。结论：通过丝裂原素诱导人单核细胞ＩＬ－２受体的表达，可以促进ＩＬ－２激活的单核细胞的杀伤活性，包括诱导靶细胞的凋亡。
AIM: To investigate the role of mitogen in the expression and cytotoxicity of interleukin 2 receptor (IL-2) in human monocytes. Methods: Human monocytes were treated with various mitogen elements and their effects on the expression and cytotoxicity of IL-2 receptor α chain in monocytes were observed. Results: Human monocytes expressed IL-2 receptor alpha chain 24 hours after treatment with Plioceri (PWM) and lipopolysaccharide (LPS); phytohemagglutinin (PHA) was observed only after 48 hours of treatment Induced the expression of α-chain, while ConA had no obvious induction effect. After pretreatment with mitogen, IL-2 was added and the activity of monocyte-killing tumor cells (U937) was obviously increased in the treatment group that induced the expression of IL-2 receptor α chain. Among them, the increase of monocyte activity in PWM pretreatment group was the most significant. Flow cytometry detected that PWM can bind both monocytes and U937 target cells simultaneously. After pretreatment with PWM, the apoptosis of U937 cells (including nuclear condensation, formation of apoptotic bodies and DNA breakage) was clearly observed after the addition of IL-2. CONCLUSION: IL-2 receptor expression induced by mitogen can promote the cytotoxic activity of IL-2-activated monocytes, including the induction of apoptosis in target cells.