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目的 :探讨脑缺血再灌损伤中雌激素与ET 3,GFAP的相互关系。 方法 :采用雌性昆明小鼠卵巢切除结合脑缺血再灌的动物模型及免疫组织化学方法。动物分为 3组 :①缺血再灌组 (IR) ,双侧颈总动脉结扎 7min ,再灌 1,3,5 ,14d ;②卵巢切除 /缺血再灌组 (OVX/IR) ,在缺血再灌前 1周作卵巢切除术 ;③假手术对照组。 结果 :对照组海马CA1区可见少量ET 3和GFAP免疫阳性细胞散在分布 ,IR组和OVX/IR组各时间点阳性细胞数明显增多 (P <0 .0 1)。缺血再灌 1d时 ,OVX/IR组ET 3和GFAP阳性细胞数明显高于IR组 (P <0 .0 1) ;5d时 ,OVX/IR组和IR组ET 3和GFAP阳性细胞数达高峰 ,在时空变化上存在一致性。 结论 :雌激素可能在缺血再灌的早期参与对ET 3和GFAP的调控而起保护作用。
Objective: To investigate the relationship between estrogen and ET 3, GFAP in cerebral ischemia-reperfusion injury. Methods: The animal model of Kunming mice ovariectomy combined with cerebral ischemia-reperfusion and immunohistochemistry were used. The animals were divided into three groups: (1) ischemia-reperfusion group (IR), bilateral common carotid artery ligation for 7 min, reperfusion for 1, 3, 5 and 14 days; ovariectomized / ischemic reperfusion group One week before ischemia and reperfusion ovariectomy; ③ sham operation control group. Results: A small amount of immunoreactive cells of ET 3 and GFAP were scattered in CA1 area of control group. The numbers of positive cells in IR group and OVX / IR group were significantly increased at each time point (P <0.01). At 1 d after ischemia / reperfusion, the number of ET 3 and GFAP positive cells in OVX / IR group was significantly higher than that in IR group (P <0.01). At 5 d, the number of ET 3 and GFAP positive cells in OVX / IR group and IR group reached Peak, there is consistency in the changes in time and space. Conclusion: Estrogen may play a protective role in the regulation of ET 3 and GFAP in the early stages of ischemia-reperfusion.