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目的 :观察大鼠不同强度运动后肾脏细胞凋亡及氧自由基变化与运动性蛋白尿之间的关系 ,以期进一步阐明运动性蛋白尿的发生机制。方法 :36只雄性SD大鼠随机分为安静对照组、中等强度运动组和大强度运动组。运动后 2小时取材 ,采用原位缺口末端标记 (TUNEL)和流式细胞术分别检测大鼠肾脏凋亡细胞及Bax、Bcl- 2基因的表达 ;同时检测大鼠肾脏组织超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH -Px)、丙二醛 (MDA)含量及运动后各实验组大鼠尿蛋白含量。结果 :运动后大强度组肾脏凋亡细胞显著增加 ,凋亡调控基因Bax/Bcl - 2比值上升 (P <0 0 1) ,尿蛋白含量显著升高 ,反映肾脏组织细胞抗氧化能力的SOD及GSH -Px活性显著下降 ,而MDA含量显著升高。结论 :大强度运动造成肾脏细胞凋亡及氧自由基增加可能参与了运动性蛋白尿的发生过程。
OBJECTIVE: To observe the relationship between renal apoptosis and oxygen free radical changes and exercise-induced proteinuria in rats after exercise with different intensities to further elucidate the mechanism of exercise-induced proteinuria. Methods: Thirty-six male Sprague-Dawley rats were randomly divided into a quiet control group, a moderate-intensity exercise group and a high-intensity exercise group. The rats were sacrificed at 2 hours after exercise. TUNEL and flow cytometry were used to detect the expression of Bax and Bcl-2, respectively. The expressions of superoxide dismutase SOD, GSH-Px and malondialdehyde (MDA) contents in rats and urinary protein content of rats in each experimental group after exercise. Results: After acute exercise, the number of apoptotic cells in kidney increased significantly, the ratio of Bax / Bcl - 2 increased (P <0.01), and the proteinuria of urine increased significantly. SOD, GSH-Px activity decreased significantly, while the MDA content increased significantly. CONCLUSION: High intensity exercise induces renal cell apoptosis and increased oxygen free radicals may be involved in the development of exercise proteinuria.