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目的 成骨生长肽(osteogenic growth peptide , OGP) 是新近发现的具有促成骨作用和刺激造血的多肽类生长因子。本研究旨在观察成骨生长肽对兔成骨细胞样细胞的促成骨作用。方法 兔颅盖骨成骨细胞样细胞分成实验组和对照组培养, 实验组加成骨生长肽。分别测定细胞的Ⅰ型胶原m RNA 表达、碱性磷酸酶活性、胶原和钙含量。结果 培养7 d 时实验组细胞的Ⅰ型胶原m RNA 高度表达,而对照组细胞的Ⅰ型胶原m RNA 表达相对较低。培养7、10 d ,2 个时间点实验组细胞胶原和钙含量与相应的对照组比较, 差异均有非常显著性意义( P< 0-001) ,尤为显著的是实验组细胞的钙含量约为对照组的3 倍。培养3 、7、10 、14 、21 d ,5 个时间点实验组细胞的碱性磷酸酶活性与相应的对照组比较, 差异均有非常显著性意义( P< 0-01～0-001) , 有的实验组细胞的碱性磷酸酶活性约为对照组的3 ～4 倍。结论 成骨生长肽能增加细胞的Ⅰ型胶原m RNA 表达,增加碱性磷酸酶活性、胶原合成和钙盐沉积。因此,成骨生长肽调节成骨细胞样细胞的功能,使细胞分化,促进成骨
OBJECTIVE: Osteogenic growth peptide (OGP) is a recently discovered peptide growth factor that promotes bone formation and stimulates hematopoiesis. The purpose of this study was to investigate the osteogenic effect of osteogenic growth peptide on osteoblast-like cells in rabbits. Methods Calvarial osteoblast-like cells were divided into experimental group and control group. Experimental group was given osteogenic growth peptide. The mRNA expression of type I collagen, alkaline phosphatase, collagen and calcium in cells were measured respectively. Results Type Ⅰ collagen mRNA was highly expressed in the experimental group on day 7 after culture, while collagen Ⅰ mRNA expression in the control group was relatively low. Compared with the corresponding control group, the differences of collagen and calcium content in the experimental group at 7, 10, and 2 time points were significant (P <0-001), especially in the experimental group 3 times as the control group. At 3, 7, 10, 14, 21 d, the alkaline phosphatase activity of the experimental group was significantly different from that of the corresponding control group (P <0-01-0-001) , And some experimental groups of cells alkaline phosphatase activity of about 3 to 4 times the control group. Conclusion Osteogenic growth peptide can increase the expression of type Ⅰ collagen mRNA and increase alkaline phosphatase activity, collagen synthesis and calcium deposition. Thus, osteogenic growth peptides regulate the function of osteoblast-like cells, differentiate cells, and promote osteogenesis