Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreac-tire T cells,which are involved in autoimmune diseases.However,it is unknown whether attenuated activated healthyautologous T-cell immunization could increase anti-tumor immune responses.To this end,C57B1/6 mice were immunizedwith attenuated activated autologous T cells.The splenocytes from immunized mice showed a higher proliferative abil-ity than that from naive mice.The special phenotype analysis showed that there were more CDS+T cells and CD62L+T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro(P<0.01).Theseresults demonstrated that this immunization may activate T cells in vivo.Furthermore,the splenocytes from immunizedmice revealed resistance to activation-induced cell death(AICD)in vitro.To further study the relative genes that areresponsible for the higher proliferation and resistance to AICD,the expression of Fas/Fas ligand(FasL)and GADD45βwas measured by real-time PCR.The results indicated that GADD45β transcription was higher in the splenocytes fromimmunized mice than that in the naive mice.In addition,the Fas expression showed a parallel higher,but FasL did notchange obviously.To investigate the biologic functions induced by immunization in vivo,a tumor model was establishedby EL-4 tumor cell inoculation in C57/B1 mice.Mice receiving autologous T-cell immunization had significantly inhibitedtumor growth in vivo(P<0.01).This study implicated that immunization with attenuated activated autologous T cellsenhances anti-tumor immune responses that participate in tumor growth inhibition. Immunization with inactivated autologous T cells may induce idiotype anti-idiotypic reactions to deplete autoreac-tire T cells, which are involved in autoimmune diseases.However, it is unknown whether or not attenuated activated-heatyaututologous T-cell immunization could increase anti-tumor immune responses. To this end, C57B1 / 6 mice were immunized with attenuated activated autologous T cells. The splenocytes from immunized mice showed a higher proliferative abil-ity than that from naive mice. The special phenotype analysis showed that there was more CDS + T cells and CD62L + T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro (P <0.01). The results indicate that this immunization may activate T cells in vivo. Future Thermo, splenocytes from immunized mouse revealed resistance to activation-induced cell death (AICD) in vitro. further study the relative genes that areresponsible for higher diffusion and resistance to AICD, the expression of Fas / Fa s ligand (FasL) and GADD45β was measured by real-time PCR. The results indicated that GADD45β transcription was higher in the splenocytes fromimmunized mice than that in the naive mice. In addition, the Fas expression showed a parallel higher, but FasL did notchangearently . To investigate the biologic functions induced by immunization in vivo, a tumor model was established by EL-4 tumor cell inoculation in C57 / B1 mice. Microbial receiving autologous T-cell immunization had significantly inhibited growth in vivo (P <0.01) .This study implicated that immunization with attenuated activated autologous T cellsenhances anti-tumor immune responses that participate in tumor growth inhibition.