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目的观察沙棘对老年大鼠血浆核磁共振(NMR)谱图的影响,以探讨沙棘干预衰老的机制。方法将SD大鼠分为青年组(4月龄,n=10)、老年对照组(24月龄,n=12)及老年干预组(24月龄,n=11)。收集各组大鼠血浆进行1H-NMR检测,应用Hotelling’s T2和趋势过滤方法分析各组血浆中代谢物的变化。结果与青年组比较,老年对照组发现与衰老相关的谱峰有28个,鉴定出16种代谢物,这些代谢物主要涉及到糖代谢、氨基酸代谢和脂类代谢。与老年对照组比较,老年干预组中被沙棘逆转的且已鉴定的代谢物有葡萄糖、乳酸、丙酮酸、甘氨酸、谷氨酰胺、亚油酸、棕榈酸、油酸、丙酮和甘油10种,沙棘能明显地改变衰老进程中的代谢物轨迹。结论大鼠衰老进程中存在糖代谢、氨基酸代谢和脂类代谢紊乱,且在不同程度上均可被沙棘逆转,表明沙棘延缓衰老的机制可能与其逆转代谢紊乱有关。
Objective To observe the effect of seabuckthorn on the plasma NMR spectra of aged rats to explore the mechanism of seabuckthorn intervention on aging. Methods SD rats were divided into four groups (4 months old, n = 10), aged control group (24 months old, n = 12) and elderly intervention group (24 months old, n = 11). The plasma of rats in each group was collected for 1H-NMR. Hotelling’s T2 and trend-filter method were used to analyze the changes of plasma metabolites in each group. Results Compared with the young group, there were 28 peaks related to aging found in the aged control group, and 16 metabolites were identified. These metabolites mainly related to glucose metabolism, amino acid metabolism and lipid metabolism. Compared with the elderly control group, the elderly intervention group was reversed by sea buckthorn and the identified metabolites were glucose, lactate, pyruvate, glycine, glutamine, linoleic acid, palmitic acid, oleic acid, acetone and glycerol 10 species, Seabuckthorn can significantly alter the trajectory of metabolites in the aging process. CONCLUSIONS: There are glucose metabolism, amino acid metabolism and lipid metabolism disorder in rats aging process, and can be reversed by seabuckthorn to varying degrees, indicating that seabuckthorn anti-aging mechanism may be related to the reversal of metabolic disorders.