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目的本文主要研究硒和维生素Ｃ影响髓系与非髓系白血病细胞增殖及其细胞毒性的异同。方法体外培养髓系白血病细胞株ＨＬ６０及非髓系细胞株Ｋ５６２，培养时分别设Ｓｅ（５μｍ、８μｍ、１１μｍ）及ＶｉｔＣ（５μｍ、１０μｍ、１００μｍ）实验组及对照组，培养２４ｈ、４８ｈ、７２ｈ后分别取样，利用台盼蓝拒染法进行细胞计数，绘制细胞生长曲线，并通过噻唑蓝比色分析法研究Ｓｅ及ＶｉｔＣ对ＨＬ６０、Ｋ５６２细胞毒性。结果细胞生长曲线显示：Ｓｅ（５～１１μｍ）及ＶｉｔＣ（５～１０μｍ）对ＨＬ６０、Ｋ５６２细胞增殖均有抑制作用。ＭＴＴ结果显示：Ｓｅ（５～１５μｍ）对两种细胞均有细胞毒性。ＶｉｔＣ（５～１０μｍ）对ＨＬ６０具有细胞毒性，而ＶｉｔＣ（５～１００μｍ）对于Ｋ５６２，短时间内毒性作用较明显，随作用时间延长而减弱。结论本实验结果提示：Ｓｅ及ＶｉｔＣ对髓系和非髓系白血病细胞增殖均有抑制作用，但ＶｉｔＣ对于ＨＬ６０和Ｋ５６２作用机制是不同的。这为白血病的化学预防和临床辅助治疗提供一定的理论依据。
Purpose This paper mainly studies the similarities and differences between selenium and vitamin C on the proliferation and cytotoxicity of myeloid and non-myeloid leukemia cells. Methods HL-60 myeloid leukemia cell line and K562 non-myeloid cell line were cultured in vitro. Se (5μm, 8μm, 11μm) and VitC (5μm, 10μm, 100μm) , 72h after sampling, the use of trypan blue exclusion method for cell counting, cell growth curve was drawn, and through the thiazole blue colorimetric assay of Se and VitC on HL 60, K562 cytotoxicity. Results The cell growth curve showed that Se (5 ~ 11μm) and VitC (5 ~ 10μm) inhibited the proliferation of HL60 and K562 cells. MTT results showed that Se (5 ~ 15μm) was cytotoxic to both cells. VitC (5 ~ 10μm) is cytotoxic to HL60 while VitC (5 ~ 100μm) is more effective on K562 in a short time, but weakened with time. Conclusion The results of this experiment suggest that Se and VitC can inhibit the proliferation of myeloid and non-myeloid leukemic cells, but the mechanism of action of VitC on HL60 and K562 is different. This provides a theoretical basis for chemical prevention and clinical adjuvant therapy of leukemia.