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目的:研究VEGF、KDR的表达及微血管密度(MVD)在肺癌中的作用及其临床意义。方法:采用免疫组化方法(LSAB法)检测114例肺癌组织、癌旁组织、30例肺良性病变组织中的VEGF、KDR表达水平及MVD。结果:1)肺癌组织中VEGF、KDR表达水平显著高于肺癌旁组织和肺良性病变组织(P<0.01),肺癌旁组织显著高于肺良性组织(P<0.05);肺癌组织中MVD显著高于肺良性病变组织(P<0.01)。2)VEGF、KDR表达水平及MVD与肺癌原发肿瘤大小、淋巴结转移状态、P-TNM分期、细胞分化程度均有密切关系(P<0.01)。3)VEGF在腺癌中表达最高,鳞癌次之,腺鳞癌最低。4)VEGF、KDR、MVD三者之间呈正相关(P<0.01)。结论:VEGF、KDR及MVD参与肺癌的发生、发展、转移,可作为肺癌生物学行为恶化的一项评估指标。
Objective: To investigate the role of VEGF and KDR expression and microvessel density (MVD) in lung cancer and its clinical significance. Methods: The expressions of VEGF, KDR and MVD in 114 cases of lung cancer, adjacent non-cancerous tissues and 30 cases of benign lung lesions were detected by immunohistochemistry (LSAB). Results: 1) The expression levels of VEGF and KDR in lung cancer tissues were significantly higher than those in lung cancer tissues and benign lung tissues (P <0.01), and those in lung cancer tissues were significantly higher than those in benign lung tissues (P <0.05); MVD in lung cancer tissues was significantly higher In benign lung lesions (P <0.01). 2) The expression of VEGF, KDR and MVD were closely related to the size of primary tumor, lymph node metastasis, P-TNM stage and cell differentiation (P <0.01). 3) The highest expression of VEGF in adenocarcinoma, squamous cell carcinoma followed, the lowest adenosquamous carcinoma. 4) There was a positive correlation between VEGF, KDR and MVD (P <0.01). Conclusion: The involvement of VEGF, KDR and MVD in the occurrence, development and metastasis of lung cancer can be used as an evaluation index for the deterioration of biological behavior of lung cancer.