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为了探讨蛹期寄生蜂对寄主蛋白代谢的寄生生理效应,利用Bradford蛋白含量测定法、Western免疫印迹法及酶联免疫吸附检测法研究了棕尾别麻蝇Boettcherisca peregrina蛹被丽蝇蛹集金小蜂Nasonia vitripennis寄生后其脂肪体和血淋巴中可溶性蛋白及芳基蛋白组成与含量的变化。结果表明:寄生蛹脂肪体和血淋巴中可溶性蛋白的组成与未寄生相比基本无明显差异;不论寄生与否寄主蛹脂肪体和血淋巴中芳基蛋白亚基分子量均为80kDa,该亚基在脂肪体中未出现降解现象,而在血淋巴中仅于寄生后12h的寄主蛹中呈现2条分子量相近的Western免疫印迹带,说明其降解可能先于未寄生对照。就含量而言,寄生蛹脂肪体中可溶性蛋白含量除寄生后24h外均显著低于未寄生对照,芳基蛋白含量除寄生后48h外也均显著低于未寄生对照,其中寄生后12h的含量仅为未寄生的32.0%。寄生蛹血淋巴中可溶性蛋白含量多低于未寄生蛹,且寄生后2,12,24h的差异达显著水平;芳基蛋白的含量均有低于未寄生的趋势,其中寄生后12h的含量为未寄生的17.0%。综合认为,丽蝇蛹集金小蜂的寄生可导致寄主脂肪体和血淋巴中可溶性蛋白及芳基蛋白含量下降。
In order to investigate the parasitological effects of parasitoids on the host protein metabolism, we used Bradford assay, Western blotting and enzyme-linked immunosorbent assay (ELISA) to study the effects of Nasonia pipiens pallens (Boettcherisca peregrina) Changes of composition and content of soluble protein and aryl protein in fat body and hemolymph after vitripennis parasitization. The results showed that the composition of soluble protein in parasitized pupae and haemolymph had no significant difference compared with non-parasitized malaria parasites. The molecular weight of both the host pupae fat body and hemolymph in the host pupae was 80kDa, No degradation occurred in the fat body, whereas two Western immunoblots with similar molecular mass appeared in the hemolymph of the host pupae only 12h after parasitization, indicating that the degradation may precede the non-parasitized control. In terms of content, the content of soluble protein in the parasitized pupae was significantly lower than that in the non-parasitized control except 24h after the parasitism, and the content of the aryl protein in the parasitized pupae was significantly lower than that in the non-parasitized control after 48h Only non-parasitic 32.0%. Parasitic pupal hemolymph in soluble protein content is much lower than the non-parasitized pupae, and parasitic 2,12,24h difference was significant; aryl protein content were lower than the non-parasitized trend, which parasitized 12h content Not parasitic 17.0%. Taken together, the parasitism of Aphelenchoides pipiens pallens could result in the decrease of soluble protein and aryl protein in the host fat body and hemolymph.