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用悬汞电极，于Ｂｒｉｔｔｏｎ－Ｒｏｂｉｎｓｏｎ－ＣＨ３ＣＨＯ（ｐＨ９．５～１０．５）体系中研究了妥布霉素、丁胺卡那霉素等五种氨基糖苷类抗生素的吸附伏安行为，并讨论了其电极过程。认为该类抗生素结构上共有的伯、仲氨基与乙醛的衍生化反应产物，在一定电位下易吸附于电极表面。其不可逆还原峰的峰电位为－１．４５Ｖ（ｖｓ．ＳＣＥ）。在选定条件下，浓度在２．０×１０（－９）～２．５×１０（－７）ｇ／ｍＬ范围内均与还原峰峰电流呈良好的线性关系，检测限低至１．０×１０（－９）ｇ／ｍＬ。方法无需预处理即可用于软膏、尿样的测定，回收率在９０．３％～１１４％之间。
The adsorption voltammetric behavior of five aminoglycoside antibiotics, tobramycin and amikacin, was investigated using suspension mercury electrode in Britton-Robinson-CH3CHO (pH 9.5 ~ 10.5) The electrode process. It is considered that the derivatization reaction products of primary and secondary amino groups with acetaldehyde which are common in the structure of these antibiotics are easily adsorbed on the electrode surface under a certain potential. The peak potential of the irreversible reduction peak was -1.45 V (vs.SCE). Under the selected conditions, the concentration ranged from 2.0 × 10 (-9) to 2.5 × 10 (-7) g / mL and the reduction peak current showed a good linear relationship with the detection limit as low as 1. 0 × 10 (-9) g / mL. The method can be used for the determination of ointment and urine without pretreatment, and the recovery rate is between 90.3% and 114%.