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七十年代后期,随着层析理论的发展和高效填充剂的发现,使原有的薄层色谱分析有了进一步提高。高效薄层色谱分析法是近十年来发展起来的一种微量分析手段,它具有点样量少、展开距离短、分辨力高、检出限度低(一般可达微微克)等优点。用作高效薄层板铺层的吸附剂,其颗粒大小通常为5～7微米直径,并要求较狭的分布范围。曾对普通薄层板及高效薄层板的分离效率进行了对比,发现一般薄层板在最佳条件时的理论塔板等板高度约为30微米,而高效薄层板则可减少至12微米,这样在展开距离为3～7厘米时,高效薄层层析板的理论塔板可达数千块之多。若用较细的吸附颗粒(如5微米)来铺层,随着展开距离的增加,它的等板高度亦迅速增加,因此吸附剂粒径小,直径分布范围狭窄,对于提高分离效率具有极为重要的意义,当然影响薄层
In the late seventies, with the development of chromatographic theory and the discovery of efficient fillers, the original TLC analysis was further improved. High performance thin layer chromatography (TLC) is a trace analysis method developed in recent ten years. It has the advantages of less spotting, short distance, high resolution and low detection limit (generally up to pico grams). Used as an adsorbent for high-performance laminates, the particle size is typically 5 to 7 microns in diameter and requires a narrow distribution range. Once compared to the separation efficiency of conventional and high-performance laminates, it has been found that the theoretical plate height of a typical lamina is about 30 microns at optimal conditions and that of high-performance laminates can be reduced to 12 Microns, so that the expansion of the distance of 3 to 7 cm, the theoretical plate of high-performance thin layer chromatography plate up to thousands of pieces. If the use of thinner particles (such as 5 microns) to layer, with the expansion of the distance increases, its plate height also increased rapidly, so the adsorbent particle size is small, narrow diameter distribution, to improve the separation efficiency is extremely The important meaning, of course, affects the thin layer