Effects and possible anti-tumor immunity of electrochemotherapy with bleomycin on human colon cancer

来源 :世界胃肠病学杂志(英文版) | 被引量 : 0次 | 上传用户:liboliang1985
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
AIM: To evaluate the anti-tumor effects and possible involvement of anti-tumor immunity of electrochemotherapy (ECT) employing electroporation and bleomycin in human colon cancer xenografts in nude mice, and to establish the experimental basis for clinical application of ECT.METHODS: Forty nude mice, inoculated subcutaneously human colon cancer cell line LoVo for 3 wk, were allocated randomly into four groups: B+E+ (ECT), B+E- (administration of bleomycin alone), B-E+ (administration of electric pulses alone), and B-E- (no treatment). Tumor volumes were measured daily. The animals were killed on the 7th d, the weights of xenografts were measured, and histologies of tumors were evaluated. Cytotoxicity of spleen natural killer (NK) and lymphokine-activated killer (LAK) cells was then assessed by lactic dehydrogenase release assay.RESULTS: The mean tumor volume of group B+E+ was statistically different from the other three groups after the treatment (F= 36.80, P<0.01). There was one case of complete response, seven cases of partial response (PR) in group B+E+, one case of PR in group B+E- and group B-E+ respectively, and no response was observed in group B-E-. The difference of response between group B+E+ and the other three groups was statistically significant (χ2 = 25.67, P<0.01). Histologically, extensive necrosis of tumor cells with considerable vascular damage and inflammatory cells infiltration were observed in group B+E+. There was no statistical difference between the cytotoxicity of NK and LAK cells in the four treatment groups.CONCLUSION: ECT significantly enhances the chemosensitivity and effects of chemotherapy in human colon cancer xenografts in nude mice, and could be a kind of novel treatment modality for human colon cancer.The generation of T-cell-dependent, tumor-specific immunity might be involved in the process of ECT.
其他文献
Though diabetes is common worldwide, the proportion of cases diagnosed is not high. Nearly fifty percent of patients with diabetes mellitus (DM) in US are undia
AIM: To study the distribution and stability of antisense oligodeoxynucleotide (ASODN) in Walker-256 cells and their distribution in liver, lung and kidney tiss
以多个星级酒店为研究对象,通过查找相关资料,应用安全系统工程的理论知识,结合酒店安全现状和管理方法,从建筑结构,火灾、内部设备、安全管理等方面对危险源进行辨识,为消除
本文采用卡尔森指数法对大伙房水库富营养化现状及趋势做分析评价.分析表明:污染源主要来自水库周边的生活源及畜禽养殖,建议针对性采取综合整治措施进行治理.
小学生常常把长方形面积计算与周长计算搞混.原因在于教师只注重对公式的教学,使学生缺乏对面积的充分感知体验.教师应有效改进手段,多给学生摆一摆,数一数,猜一猜.通过充分
AIM: To investigate the survivin gene expression in human hepatocellular carcinoma cell line SMMC-7721 and the effects of survivin gene RNA interference (RNAi)
本文作者根据多年的语文教学经验,阐述了在阅读环节里学生挖掘创新意识的方法.
AIM: To investigate IL-1β+3 594 in the 5th intron, IL-10-1 082 and CD14-159 polymorphisms in patients with acute pancreatitis (AP) and septic shock.METHODS: Th
AIM: To investigate β-catenin expression in patients with intestinal metaplasia, and to look for a possible relationship between β-catenin expression and eith
对变频技术在增压器试验中的应用情况进行了介绍,对应用前后的不同之处进行了比较分析,指出提升增压器出厂试验台性能的重要性.