目的　探讨单核细胞 (Mo)NF κB核易位及其检测方法 ,了解脂多糖 (LPS)对NF κB的活化作用。方法　采用免疫组化方法和凝胶电泳迁移率改变分析 (EMSA)法。结果　Mo免疫组化结果 ,胞浆组化染色强阳性 ,其胞核染色弱阳性 ;LPS刺激Mo后 ,胞核组化染色显强阳性。EMSA示LPS刺激组的自显影密度远高于正常对照组 (P <0 .0 1 )。结论　Mo受LPS刺激后 ,免疫组化方法证实在完整细胞中NF κB核易位并活化 ;EMSA证明LPS刺激后 ,NF κB核易位大量增加 ,与κB序列结合活力大为增强。 Objective To investigate the nuclear translocation of monocyte (NF) κB and its detection methods to understand the activation of NF κB by lipopolysaccharide (LPS). Methods Immunohistochemistry and electrophoretic mobility shift assay (EMSA) were used. Results Mo immunohistochemical results showed that cytoplasm staining was strongly positive and weakly positive staining of nuclei. After LPS stimulation of Mo, the staining of nuclei was strongly positive. EMSA showed that the self-imaging density of LPS stimulation group was much higher than that of normal control group (P <0.01). Conclusion After LPS stimulated by LPS, the nuclear translocation and activation of NF κB were confirmed by immunohistochemistry. EMSA demonstrated that the nuclear translocation of NF κB increased significantly after LPS stimulation, and the binding activity with κB increased greatly.