检测了抗迟缓爱德华菌单抗对牙鲆的保护性。从１０株自制单抗中筛选出一株对牙鲆具有较强保护性的单抗３Ｆ７。与仅以该菌感染的对照组相比，该抗体可显著提高牙鲆感染迟缓爱德华菌后的存活率。在以每条鱼０．１ｍＬ（１０９ＣＦＵ）菌量做攻击保护时，其保护率可达８０％。另外，利用所制单克隆抗体以免疫组织化学ＳＡＢＣ法检查了迟缓爱德华菌经腹腔感染牙鲆鱼后细菌的侵染途径，结果显示牙鲆对该菌易感的器官为肝、肾、牌等，而胃、肠等器官并不易被感染。 The protective effect of anti-delay Edwardella monoclonal antibody on Japanese flounder was tested. Ten monoclonal antibodies were screened from a monoclonal antibody to Paralichthys olivaceus 3F7. Compared with the control group infected only with this bacterium, the antibody significantly increased the survival rate of the turbot infected with S. edwardsiana. In the protection of 0.1% (109CFU) bacteria per fish, the protection rate can reach 80%. In addition, the use of the monoclonal antibody immunohistochemical SABC method to check the delay after infection of E. coli larvae infection of bacteria flounder Paralichthys olivaceus flounder Paralichthys olivaceus organism susceptible organs of the liver, kidney, card, etc. , While the stomach, intestine and other organs are not easily infected.