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目的构建人参Pg MYB4基因表达载体,并研究该基因对拟南芥的抗旱功能。方法采用RT-PCR技术克隆人参Pg MYB4基因,构建表达载体,通过农杆菌介导的花序浸蘸法将其转化到拟南芥中,获得转基因拟南芥,以野生型拟南芥作对照,检测植株与抗旱相关的生理指标。结果获得的人参Pg MYB4基因全长为735 bp,编码245个氨基酸,预测其相对分子质量为27 914;在干旱胁迫条件下,转基因拟南芥的生长状态明显优于野生型拟南芥,与野生型相比,转基因拟南芥叶片相对叶绿素量降低幅度小、脯氨酸量显著升高、水丢失率较低。结论人参Pg MYB4基因具有抗干旱胁迫的能力。
Objective To construct the gene expression vector of Pg MYB4 and study its drought resistance function in Arabidopsis thaliana. Methods The gene of Pg MYB4 was cloned by RT-PCR. The expression vector was constructed and transformed into Arabidopsis thaliana by the method of inflorescence digestion mediated by Agrobacterium tumefaciens. The transgenic Arabidopsis thaliana was obtained. Detect the physiological indexes related to drought resistance. Results The total length of the Pg MYB4 gene was 735 bp, encoding 245 amino acids. The predicted molecular weight of the MYB4 gene was 27 914. Under drought stress, the transgenic Arabidopsis thaliana grew significantly better than the wild type Arabidopsis thaliana, Compared with the wild-type, the relative chlorophyll content of transgenic Arabidopsis leaves decreased slightly, proline content increased significantly, and water loss rate was lower. Conclusion Ginseng Pg MYB4 gene has the ability of anti-drought stress.