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目的：探讨应用RNA干扰技术沉默Aurora-A基因表达,研究其对人卵巢癌SKOV3细胞增殖的抑制作用。方法：设计合成两对特异性针对Aurora-A基因的Oligo siRNA,转染至SKOV3细胞中,采用RT-PCR和Western blot检测Aurora- A mRNA和蛋白表达情况,同时利用MTT试验和流式细胞仪观察转染后细胞增殖抑制和凋亡情况。结果：转染Oligo siRNA后,SKOV3细胞Aurora-A mRNA表达受抑制(P＜0．01),蛋白表达水平降低；细胞增殖的抑制率和细胞凋亡率明显增高(P＜0．05,P＜0．01)。结论：体外合成的特异性针对Aurora-A基因的Oligo siRNA对卵巢癌细胞株SKOV3中Aurora-A基因表达和细胞增殖均有明显抑制作用,为进一步研究Aurora-a基因的功能提供了实验基础。
OBJECTIVE: To investigate the effect of Aurora-A gene silencing on the proliferation of human ovarian cancer cell line SKOV3 by RNA interference. Methods: Two pairs of oligonucleotides targeting Aurora-A gene were designed and synthesized and transfected into SKOV3 cells. The expression of Aurora-A mRNA and protein was detected by RT-PCR and Western blot. MTT assay and flow cytometry The inhibition of cell proliferation and apoptosis after transfection were observed. Results: The expression of Aurora-A mRNA in SKOV3 cells was inhibited (P <0.01) and the expression of Aurora-A mRNA was decreased after transfection with Oligo siRNA; the cell proliferation inhibition rate and apoptosis rate were significantly increased (P <0.05, P <0.01). CONCLUSION: Oligo siRNA targeting Aurora-A gene synthesized in vitro has significant inhibitory effect on Aurora-A gene expression and cell proliferation in ovarian cancer cell line SKOV3, providing an experimental basis for further study on the function of Aurora-a gene.