论文部分内容阅读
目的建立巴戟天药材寡糖化学成分的高效阴离子交换色谱-脉冲安培电化学检测器(HPAEC-PAD)特征指纹图谱,为制定巴戟天药材质量标准提供参考数据。方法利用HPAEC-PAD,色谱条件为,Hamilton RCX-10(4.1 mm×250 mm,7μm),100 mmol.L-1 NaOH溶液(A)-100 mmol.L-1 NaOH,500 mmol.L-1 NaAc混合溶液(B)流动相,梯度洗脱,流速为0.8 mL.min-1,柱温为30℃,检测电压0.1V,分析时间为40 min。结果建立了巴戟天药材寡糖化学成分的HPAEC-PAD指纹图谱,并标定出巴戟天药材20个共有峰,其中3个峰经与对照品比对为蔗果三糖、耐斯糖(nystose)、1F-果呋喃糖基耐斯(1F-fructo-furanosylnyslose),经指纹图谱系统解决方案软件(ChromafingerTM)生成共有模式,并进行相似度和主成分分析。不同产地巴戟天药材均含有以上共有峰,但在共有峰的峰高上有差异,并可用于区别巴戟天及其常见混伪品。结论利用该方法建立的巴戟天寡糖类化学成分的指纹图谱特征性和专属性强,且该方法快速、灵敏、可靠,可用于控制巴戟天药材的质量。
OBJECTIVE To establish a HPLC fingerprint of HPAEC-PAD for the chemical composition of medicinal oligosaccharides of Morinda officinalis, and provide reference data for establishing the quality standard of Morinda officinalis. Methods HPAEC-PAD was used. The chromatographic conditions were Hamilton RCX-10 (4.1 mm × 250 mm, 7 μm), 100 mmol·L -1 NaOH solution (A) -100 mmol·L -1 NaOH, 500 mmol·L -1 The mobile phase of NaAc mixed solution (B) eluted with a gradient of 0.8 mL.min-1. The column temperature was 30 ℃, the detection voltage was 0.1V and the analysis time was 40 min. Results The chemical composition of the medicinal plants of Morinda officinalis, HPAEC-PAD, was established and 20 common peaks of Radix Morinda officinalis were screened. Among them, the three peaks were compared with the control, nystose, 1F-fructo-furanosylnyslose, and a consensus model and principal component analysis were generated using the ChromafingerTM software. Morinda officinalis from different areas contain the above common peaks, but there are differences in the peak of common peaks, and can be used to distinguish Morinda officinalis and its common dummies. Conclusion The fingerprint of the chemical composition of Morinda officinalis Oliv. Was established by this method is characterized by its specificity and specificity. The method is rapid, sensitive and reliable and can be used to control the quality of Morinda officinalis.