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目的探讨一氧化氮在梗阻性黄疸患者肝损伤中的作用及其意义。方法选取阻塞性黄疸患者 30例作为实验组 ,另选取同期住院的单纯性胆囊结石患者 30例作为对照组。观察各组血清一氧化氮 (NO)、肿瘤坏死因子 (TNF)、谷草转氨酶 (GOT)、谷丙转氨酶 (GPT)、胆红素 (TBil)、白蛋白及血浆内毒素水平变化。取阻塞性黄疸患者肝组织作成 10 %生理盐水匀浆 ,计算每克 (湿重 )肝组织NO -2 含量 ;取材同上 ,制作石蜡切片HE染色 ,计算机图像分析仪计算HE染色切片肝细胞坏死面积。相关分析肝组织坏死面积与肝组织NO含量的相关关系。结果实验组一氧化氮、内毒素、肿瘤坏死因子水平较对照组差异有显著性 (P <0 .0 1) ;肝组织坏死面积与NO含量之间呈显著正相关 ;血清NO含量与血清谷草转氨酶 (GOT)、谷丙转氨酶 (GPT)、胆红素 (TBil)之间呈显著正相关。结论梗阻性黄疸患者一氧化氮水平显著升高 ,体内一氧化氮水平的高低与肝损伤程度相平行 ,抑制或促进一氧化氮的合成与释放可能对肝脏起保护作用 ,检测体内一氧化氮水平可成为了解梗阻性黄疸患者肝损伤程度的重要指标之一。
Objective To investigate the role of nitric oxide in liver injury in patients with obstructive jaundice and its significance. Methods Thirty patients with obstructive jaundice were selected as the experimental group. Thirty patients with simple gallstone in the same period were selected as the control group. The levels of serum NO, TNF, GOT, GPT, TBil, albumin and plasma endotoxin in each group were observed. The liver tissue of patients with obstructive jaundice was homogenized with 10% saline to calculate the content of NO-2 per gram (wet weight) of liver tissue. The above materials were made into paraffin sections and stained with hematoxylin and eosin (HE) . Correlation analysis of liver necrosis area and liver tissue NO content. Results The levels of nitric oxide, endotoxin and tumor necrosis factor in the experimental group were significantly lower than those in the control group (P <0.01). There was a significant positive correlation between the necrosis area and the content of NO in the experimental group. There was a significant positive correlation between GOT, GPT and TBil. Conclusions In patients with obstructive jaundice, the level of nitric oxide is significantly increased. The level of nitric oxide in the body is in parallel with the degree of liver injury. Inhibition or promotion of nitric oxide synthesis and release may play a protective role on the liver. Nitric oxide Can become one of the important indicators to understand the degree of liver injury in patients with obstructive jaundice.