人胰岛素是治疗糖尿病不可缺少的药品,如何制备较大量的人胰岛素提供临床应用,是一个重要问题。近几年来,Johanson等人从酒酵母(saccharomyces cerirlsiae)中提取羧肽酶Y,通过此酶的转肽作用,成功地合成了各种短肽和长肽。在此基础上,又用此酶将猪胰岛素B半合成为人胰岛素。 Human insulin is indispensable for the treatment of diabetes drugs, how to prepare a larger amount of human insulin to provide clinical applications, is an important issue. In recent years, Johanson et al. Extracted carboxypeptidase Y from saccharomyces cerirlsiae, and successfully synthesized various short peptides and long peptides by the transpeptidation of this enzyme. On this basis, this enzyme is used to semi-synthesize porcine insulin B as human insulin.