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目的:探讨2种方式移植人脐带间充质干细胞(UCMSC)对TNBS诱导的炎症性肠病小鼠模型的治疗作用。方法:分离培养人UCMSC,并通过一次性腹腔注射TNBS制备炎症性肠病动物模型,采用尾静脉注射和腹腔注射2种方式移植人UCMSC进行治疗,4周后处死,观察动物的死亡率、体重改变及结肠炎症变化,并进行大体和病理评分。结果:与对照组相比,尾静脉和腹腔注射移植UCMSC组动物死亡率下降,体重恢复较早,结肠病变大体评分和病理评分均显著改善;2种方式移植组之间的评分无显著差异。结论:尾静脉和腹腔移植人UCMSC对TNBS诱导的炎症性肠病小鼠模型均有治疗作用。
Objective: To investigate the therapeutic effects of transplanted human umbilical cord mesenchymal stem cells (UCMSCs) in two ways on mouse models of inflammatory bowel disease induced by TNBS. Methods: Human UCMSC was isolated and cultured. The animal model of inflammatory bowel disease was established by one intraperitoneal injection of TNBS. Human UCMSC was transplanted by both caudal vein injection and intraperitoneal injection. After 4 weeks, the mice were sacrificed. The mortality and body weight Changes and changes in colonic inflammation, and gross and pathological score. Results: Compared with the control group, the mortality rate of UCMSC group was lower than that of the control group, the body weight recovered earlier, and the general and pathological scores of colon lesions were significantly improved. There was no significant difference between the two groups in the grading. Conclusion: The tail vein and abdominal transplanted human UCMSC have a therapeutic effect on TNBS-induced mouse model of inflammatory bowel disease.