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目的观察小承气汤对支气管炎模型大鼠肺与结肠组织水通道蛋白(aquaporin,AQP)的调节效应,探讨“肺病及肠与泻肠清肺”之“水湿”关链的可能机制。方法 SD大鼠60只,随机分为空白组、模型组、观察组,每组20只。模型组及观察组单纯烟熏法70天建模。检测大鼠肺功能、血气分析,计算结肠湿重指数,免疫组化法、Western blot法检测大鼠肺组织AQP1蛋白与结肠组织AQP4蛋白表达。结果与空白组比较,模型组大鼠肺顺应性降低、气道阻力增加(P<0.05),肺功能降低,血气分析中PO2降低、PCO2升高(P<0.05),大鼠肺组织AQP1及结肠组织AQP4表达明显下降(P<0.05);与模型组比较,观察组大鼠气道阻力下降,肺顺应性升高(P<0.05);低氧高酸状态(PO2/PCO2/p H)显著改善(P<0.05),肺组织AQP1表达显著升高(P<0.05)。结论肺组织AQP1和结肠组织AQP4可能是“肺病及肠”病理传变中“水湿”的链点之一,亦是“泻肠清肺”功效的新位点,属“肺与大肠相表里”的新证据。
Objective To observe the effect of Xiao Cheng Qi Decoction on the regulation of aquaporin (AQP) in the lung and colon tissues of rats with bronchitis, and to investigate the effect of Xiao Cheng Qi Decoction on aquaporin (AQP) Possible mechanism. Methods Sixty SD rats were randomly divided into blank group, model group and observation group, with 20 rats in each group. Model group and observation group were smoked only for 70 days. Lung function, blood gas analysis, colon wet weight index, immunohistochemistry and Western blot were used to detect the expression of AQP1 protein and AQP4 protein in colon tissue. Results Compared with the blank group, the lung compliance, the airway resistance and the pulmonary function decreased, the PO2 decreased and the PCO2 increased (P <0.05) in the model group, the AQP1 and Compared with the model group, the airway resistance and the lung compliance of the rats in the observation group were significantly increased (P <0.05); the levels of PO2 / PCO2 / p H in the colon tissue were significantly decreased (P <0.05) (P <0.05). The expression of AQP1 in lung tissue was significantly increased (P <0.05). Conclusion AQP1 in lung tissue and AQP4 in colon tissue may be one of the link points in the pathogenesis of “lung disease and intestine” and “water wetness”, and also a new site for the efficacy of “ ”Lung and large intestine " in the new evidence.