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目的探索肿瘤坏死因子α（ＴＮＦα）诱导人大肠癌细胞凋亡的可能性及其发生规律．方法用细胞体外培养方法，以ＴＮＦα１×１０８Ｕ／Ｌ作用人大肠癌Ｌｏｖｏ细胞０ｈ～４８ｈ，经ＨＥ染色和Ｈｏｅｃｈｓｔ３３２５８荧光染色，光镜观察细胞的形态改变并计算凋亡百分率；ＤＮＡ抽提、琼脂糖凝胶电泳，检测ＤＮＡ断裂情况．结果随作用时间由０，１２，２４，３６延长到４８ｈ，Ｌｏｖｏ细胞渐出现明显的凋亡形态学改变，凋亡百分率分别为２０４％，６５９％，２３８９％，６０４７％，９２６６％（Ｐ＜００１）；作用４８ｈ，ＤＮＡ电泳出现梯形条带．结论ＴＮＦα能够诱导人大肠癌Ｌｏｖｏ细胞凋亡，并存在时间效应．
Objective To explore the possibility and occurrence of apoptosis of human colorectal cancer cells induced by tumor necrosis factor α (TNFα). Methods Human colon cancer cells Lovo cells were treated with TNFα1×108 U/L for 0 h～48 h with cell culture in vitro. HE staining and Hoechst 33258 fluorescence staining were used to observe the morphological changes of cells and calculate apoptosis percentage by light microscopy; DNA extraction, agarose Gel electrophoresis to detect DNA fragmentation. Results With the prolongation of the action time from 0, 12, 24, 36 to 48 h, Lovo cells gradually showed obvious apoptotic morphological changes. The percentage of apoptosis was 2.4%, 6.9%, 23.9%, and 60%, respectively. 47%, 92 66% (P<001); After 48h, trapezoidal bands appeared in DNA electrophoresis. Conclusion TNFα can induce apoptosis of human colon cancer Lovo cells with time effect.