论文部分内容阅读
目的:初步探讨背侧抑制性轴突导向蛋白即draxin对鸡胚后脑23C10阳性神经元轴突投射过程中的调控作用。方法:对HH13-14发育阶段的正常鸡胚后脑内进行跨膜型和分泌型draxin质粒转染,通过免疫组织化学染色方法,观察HH25-26发育阶段鸡胚23C10阳性神经元轴突的投射情况;应用活组织离体培养方法,检测draxin对鸡胚后脑神经元轴突生长的影响。结果:跨膜型draxin过表达后,有较多23C10阳性神经元的轴突异常投射到同侧后脑背侧部;分泌型draxin过表达后,有少部分23C10阳性神经元的轴突异常投射到同侧后脑背侧部;正常鸡胚和空白质粒过表达对照组均未观察到异常投射的神经元轴突。鸡胚后脑活组织离体培养中,draxin显著抑制体外培养组织内树突的生长,而对照组可见体外培养的组织内有大量树突形成。结论:draxin对鸡胚后脑内23C10阳性神经元的轴突投射具有抑制性导向作用。
OBJECTIVE: To investigate the role of draxin, a dorsal suppressive neurotrophic factor in the axon projection of 23C10-positive neurons in chick embryo brain. Methods: The trans-membrane-type and secretory draxin plasmids were transfected into the normal chick embryo brain in the developmental stage of HH13-14. The projections of 23C10-positive neurons in chick embryo HH25-26 were observed by immunohistochemical staining ; The application of tissue culture in vitro to detect the draxin on neurite outgrowth of chicken embryo growth. RESULTS: After transmembrane draxin overexpression, more axons of 23C10 positive neurons were projected to the dorsal part of ipsilateral hindbrain. After the draxin overexpression, a few axons of 23C10 positive neurons were projected to Ipsilateral posterior dorsal hindbrain; normal embryos and blank plasmid overexpression control group were not observed abnormally projected neuronal axons. In vitro culture of chick embryo brain tissue, draxin significantly inhibited the growth of dendrites in cultured tissue, while the control group showed a large number of dendrites formed in the tissue culture. Conclusion: draxin can inhibit the axon projection of 23C10 positive neurons in the chick embryo brain.