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目的:研究白藜芦醇(Resveratrol,Res)对脓毒症大鼠急性肺损伤(Acute lung injury,ALI)的保护作用及对ALI大鼠肺组织中高迁移率族蛋白(high mobility group box 1 protein,HMGBl)及Toll样受体4(Toll-like receptor 4,TLR4)mRNA表达的影响。方法:将大鼠随机分为假手术组(Sham组)、脓毒症组(Sep组)和白藜芦醇组(Res组),采用盲肠结扎穿刺术(Cecal ligation and puncture,CLP)制备脓毒症相关性ALI大鼠模型,造模1 h后,Res组给予40mg/kg,Sham和Sep组给予等量生理盐水,均经股静脉注射。各组分别于2、6、12和24h,取大鼠肺组织,HE染色观察病理形态学变化,RT-PCR检测脓毒症大鼠各器官组织HMGB1及TLR4的mRNA的动态表达变化。结果:Res组在造模后12 h,肺组织病理变化与Sep组相比有所减轻,24 h Res作用达到最强,各种病理改变明显减轻,部分肺组织开始接近正常形态;Res组与Sepsis组比较,肺组织HGMB1及TLR4 mRNA表达明显下调。结论:Res可以通过下调肺组织HGMB1及TLR4 mRNA表达减轻脓毒症诱导的肺损伤。
Objective: To investigate the protective effect of resveratrol (Res) on acute lung injury (ALI) in septic rats and the effect of high mobility group box 1 protein , HMGBl), and Toll-like receptor 4 (TLR4) mRNA expression. Methods: The rats were randomly divided into sham operation group (Sham group), sepsis group (Sep group) and resveratrol group (Res group). Cecal ligation and puncture (CLP) After 1 h of modeling, the rats in the Res group were given 40 mg / kg of ALI rat model. The rats in Sham and Sep groups were given the same amount of saline, all of which were injected through the femoral vein. The rats were sacrificed at 2, 6, 12 and 24 hours respectively. Pathological changes were observed by HE staining. The mRNA expression of HMGB1 and TLR4 in various tissues of septic rats were detected by RT-PCR. Results: In Res group, the pathological changes of lung tissue were relieved compared with those of Sep group at 12 h after model making. The effect of Res reached the strongest at 24 h, and all pathological changes were alleviated. Some lung tissues began to approach normal morphology. Res group Sepsis group, lung tissue HGMB1 and TLR4 mRNA expression was significantly down-regulated. Conclusion: Res can attenuate sepsis-induced lung injury by down-regulating the expression of HGMB1 and TLR4 mRNA in lung tissue.