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目的 探讨早期应用不同剂量氨基胍对内毒素休克模型兔肾功能的保护作用及氨基胍治疗的剂量、时间依赖性。方法 4 0只新西兰白兔麻醉后被随机分成 5组 :对照组、内毒素组、氨基胍一组、二组、三组。除对照组外 ,其他四组每只兔静脉推注大肠杆菌O5 5B5内毒素 (LPS) 4 0 0 μg/kg ,当平均动脉压下降为原来的 30 %时 ,休克诱导成功。氨基胍一、二、三组每只兔分别静脉注射 30、5 0、10 0mg/kg的氨基胍 ,对照组及内毒素组给予相应剂量的生理盐水。在注射内毒素前 (T0 )、休克时(T)、休克后第 1(T1)、2 (T2 )、3(T3 )、4 (T4)、5 (T5)、6 (T6)小时记录尿量 ,在T、T2 、T4、T6时间点测定血硝酸盐、亚硝酸盐 (NO-3 /NO-2 ,NO的代谢产物 )、尿素氮 (BUN)、肌酐 (Scr)、尿视黄醇结合蛋白(RBP)。结果 LPS可引起血NO-3 /NO-2 、BUN、Scr、尿RBP显著升高 [分别从T点的 (47± 5 ) μmol/L、(5 8± 1 5 )mmol/L、(41± 10 ) μmol/L、(2 4 0± 6 1)ng/L ,升高至T6点的 (16 0± 18) μmol/L、(15 5±1 8)mmol/L、(16 6± 2 3) μmol/L、(15 80± 180 )ng/L ,P均 <0 0 1]。尿量显著减少 [从T0 点的 (17 6± 2 8)ml降低到T6点的 (1 3± 0 6 )ml,P <0 0 1]。三个氨基胍组均可减轻NO-3 /NO-2 、BUN、SCr、RBP升高?
Objective To investigate the protective effects of aminoguanidine on the renal function of rabbits with endotoxin shock and the dose and time dependence of aminoguanidine in early stage. Methods 40 New Zealand White rabbits were randomly divided into five groups: control group, endotoxin group, aminoguanidine group, two groups and three groups. In addition to the control group, each of the other four groups was intravenously injected with 450 μg of lipopolysaccharide (LPS) of Escherichia coli at a dose of 400 μg / kg. When the mean arterial pressure was reduced to 30% of the original value, shock induction was successful. Aminoguanidine one, two and three groups were given intravenous injection of 30, 50 and 100 mg / kg aminoguanidine respectively. The control group and endotoxin group were given the corresponding doses of normal saline. Urine was recorded before (T0), shock (T), 1 (T1), 2 (T2), 3 (T3), 4 (T4), 5 (T5) and 6 (T6) hours after shock The levels of blood nitrate, nitrite (NO-3 / NO-2, NO metabolite), BUN, creatinine (Scr), urinary retinal Binding protein (RBP). Results LPS significantly increased the levels of NO-3 / NO-2, BUN, Scr and urinary RBP [(47 ± 5) μmol / L, (51.8 ± 1.5) mmol / L, (16 ± 18) μmol / L and (15 ± 5 ± 18) mmol / L and (16 ± 6) ng / L and 2 3) μmol / L and (15 80 ± 180) ng / L respectively, all P <0 01]. Urine volume decreased significantly (from (17 6 ± 2 8) ml at T 0 to (13 ± 0 6) ml at T 6, P 0 01). Three aminoguanidine groups could reduce NO-3 / NO-2, BUN, SCr, RBP increased?