Observation on Double Fertilization and Early Embryonic Development in Autotetraploid Polyembryonic

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The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy.Some abnormalities including degenerated ovary,abortive embryo sac,single fertilization,double-ovule and double-embryo and so on.were found during double fertilization and embryo development in IR36-Shuang.The rate of the abnormalities was 46.67%in IR36-Shuang,significantly higher than that in the control,an autotetraploid rice line IR36-4X(33.00%) .Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang. The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Domestic abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double- embryo and so on. were found during double fertilization and embryo development in IR36-Shuang. rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X (33.00%) . Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.
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