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目的探讨多氯联苯(PCBs)153通过诱导P450酶活力对苯并(a)芘[B(a)P]遗传毒性的影响。方法PCB153设3个剂量组(1、10、100μmol/L),B(a)P设1个剂量组(50μmol/L),DMSO为溶剂对照组,3-甲基胆蒽(3-MC)为阳性对照组。以不同浓度的PCB153(1、10、100μmol/L)染毒HepG2细胞48h后再与B(a)P联合染毒24h。通过荧光分光光度法测定HepG2细胞CYP1A1酶活力(EROD)。同时采用胞质分裂阻滞法微核试验(CBMNT)分析细胞的微核,计算微核率和核分裂指数(NDI)。结果与溶剂对照组相比,1、10、100μmol/L的PCB153和50μmol/L的B(a)P单独染毒均可诱导EROD活力增加,差异有统计学意义(P<0.05)。与溶剂对照组比较,100μmol/L的PCB153和50μmol/L的B(a)P可诱导微核率显著升高,差异有统计学意义(P<0.01)。与B(a)P单独染毒组比较,B(a)P和PCB153联合染毒时,EROD活力和微核率均显著升高,差异有统计学意义(P<0.05或P<0.01)。与溶剂对照组比较,100μmol/L的PCB153和50μmol/L的B(a)P及所有联合染毒组均使HepG2细胞NDI明显降低,差异有统计学意义(P<0.05或P<0.01)。结论PCB153对B(a)P的遗传毒性作用具有一定的促进作用,这种促进可能与PCB153诱导P450酶活力有关。
Objective To investigate the effects of PCBs 153 on the genotoxicity of benzo (a) pyrene [B (a) P] induced by P450 enzyme activity. Methods PCB153 was divided into 3 groups (1, 10, 100μmol / L), B (a) P group, 50μmol / L DMSO group and 3-methylcholanthrene group For the positive control group. HepG2 cells were exposed to different concentrations of PCB153 (1, 10 and 100 μmol / L) for 48 h and then incubated with B (a) P for 24 h. The CYP1A1 enzyme activity (EROD) of HepG2 cells was determined by fluorescence spectrophotometry. At the same time, the micronucleus test (CBMNT) was used to analyze the micronucleus and calculate the micronucleus rate and mitotic index (NDI). Results Compared with the solvent control group, PCB153 at 1, 10, 100μmol / L and B (a) P at 50μmol / L alone could induce the increase of EROD activity with statistical significance (P <0.05). Compared with the solvent control group, the micronucleus rate of PCB153 (100μmol / L) and B (a) P of 50μmol / L were significantly increased (P <0.01). Compared with B (a) P alone group, EROD activity and micronucleus rate of B (a) P and PCB153 were significantly increased (P <0.05 or P <0.01). Compared with the solvent control group, the NDI of HepG2 cells was significantly decreased in both PCB153 (100μmol / L) and B (a) P at 50μmol / L and all the combination groups. The difference was statistically significant (P <0.05 or P <0.01). Conclusion PCB153 can promote the genotoxicity of B (a) P to a certain extent, which may be related to the induction of P450 enzyme activity by PCB153.