利用Ｔｒｉｓ－缓冲液－甘油提取法对硒酵母的化学形态进行了分析，并与透析法进行比较。结果表明，经过特殊工艺生产的硒酵母虽然总硒含量达到４０００μｇ／ｇ以上，但其中５０％以上的硒为硒蛋氨酸形态的蛋白质或其它大分子态硒，无机态硒仅占３％左右，而游离态硒只占０．０１％。说明硒酵母有广阔的开发前景，同时也为硒制剂提供了适宜的质量监测方法。 The chemical form of selenium yeast was analyzed by Tris-buffer-glycerol extraction and compared with dialysis method. The results showed that, although the total selenium content of the selenium yeast produced by the special process reached more than 4000μg / g, more than 50% selenium was the selenomethionine-form protein or other macromolecular selenium, and the inorganic selenium accounted for only about 3% Free selenium only 0.01%. Selenium yeast shows broad prospects for development, but also for selenium preparations provide an appropriate quality monitoring methods.