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目的:从酒精性肝损伤与肠内毒素渗漏的病理联系,探讨乌药保护酒精性肝损伤的作用和机理。方法:采用SD大鼠灌胃给予体积浓度50%的白酒,每日1次,灌胃容积1.2 m L/100g,连续33 d建立酒精性肝损伤模型,造模的同时灌胃给予乌药不同提取物连续33 d。末次给药后禁食12 h,一次性灌胃给予10 mg/kg LPS,3.5h后下腔静脉及门静脉采血,测定血清ALT、AST、TBIL、TNF-α、IL-6、IL-1β、LPS水平。结果:模型组ALT、AST、TBIL、TNF-α、IL-6、IL-1β显著升高;模型组门静脉血清内毒素水平显著升高,提示存在肠通透性增高;较之模型组,乌药组的上述指标均有显著改善;肝损伤指标及炎症因子水平与门静脉内毒素水平均正相关。结论:乌药可以抑制酒精引起的肠通透性增加,减轻内毒素渗漏,从而减轻肝损伤。
Objective: To investigate the mechanism and mechanism of the action of UFG in protecting alcoholic liver injury from the pathological connection between alcoholic liver injury and intestinal endotoxin leakage. Methods: Sprague-Dawley rats were administered intragastrically with 50% volume liquor, once a day, with an intragastric volume of 1.2 m L / 100g for 33 consecutive days to establish alcoholic liver injury model. Extracts for 33 consecutive days. After the last administration, rats were fasted for 12 h and given a single oral dose of 10 mg / kg LPS. Blood samples were obtained from the inferior vena cava and the portal vein 3.5 hours later. Serum levels of ALT, AST, TBIL, TNF-α, IL-6 and IL- LPS level. Results: The levels of ALT, AST, TBIL, TNF-α, IL-6 and IL-1β in the model group were significantly increased. The level of endotoxin in the portal vein of the model group was significantly higher than that in the untreated group The above indexes in the medicine group all improved significantly. The indexes of liver injury and inflammatory cytokines were positively correlated with the levels of portal venous endotoxin. Conclusion: Wuyao can inhibit alcohol-induced intestinal permeability increase, reduce endotoxin leakage, thereby reducing liver damage.