为从蛋白质水平揭示甜高粱适应苏打盐碱胁迫的分子机制,以耐性较强的甜高粱品种M-81E为材料,通过双向电泳和质谱技术鉴定差异表达蛋白质并进行生物信息学分析,同时测定苏打盐碱胁迫下植株抗氧化酶系统活性动态变化。结果表明:在处理条件下,所鉴定的44个差异表达蛋白质有30个上调表达,14个下调表达,主要涉及物质和能量代谢、信号转导及转录调控等功能;GO分析表明,差异蛋白质主要参与氧化还原生物学过程;KEGG富集分析表明,差异蛋白质主要参与碳代谢、光合生物碳固定、糖酵解和次级代谢物生物合成等代谢途径;蛋白质间相互作用直接或间接参与各种代谢通路。苏打盐碱胁迫4 h后,处理组SOD和CAT活性显著下降(P<0.05),GSH-Px酶活性无明显变化,而POD活性极显著升高(P<0.01),并且此时丙二醛和H_2O_2含量较对照组极显著降低(P<0.01),推测在胁迫初期POD是清除活性氧的主效酶;各种抗氧化酶在胁迫16 h时活性呈现不同程度下降趋势。当胁迫24 h时,各种酶活性仍显著高于对照组,但丙二醛和H_2O_2含量剧增。 In order to reveal the molecular mechanism of sweet sorghum adaptation to soda-salinia stress at the protein level, the differential expression protein was identified by two-dimensional electrophoresis and mass spectrometry using the sorghum cultivar M-81E with high tolerance and bioinformatics analysis, Dynamic Changes of Antioxidant Enzyme System Activities in Saline - Alkali Stress Plants. The results showed that under the treatment conditions, the identified 44 differentially expressed proteins were up-regulated 30 and down-regulated 14, mainly related to the function of substance and energy metabolism, signal transduction and transcriptional regulation; GO analysis showed that the differential proteins mainly Participate in the redox biological process; KEGG enrichment analysis showed that the differential proteins are mainly involved in metabolic pathways such as carbon metabolism, photosynthetic carbon fixation, glycolysis and secondary metabolite biosynthesis; protein-protein interactions directly or indirectly involved in various metabolic path. The activities of SOD and CAT were significantly decreased (P <0.05) and the activities of GSH-Px were not significantly changed, but the activities of POD were significantly increased (P <0.01) after treated with saline-alkali for 4 h, (P <0.01). It was speculated that POD was the main active enzyme to scavenge reactive oxygen species (ROS) at early stage of stress. The activity of various antioxidant enzymes showed a downward trend in different degrees after 16 h of stress. When 24 h stress, the activity of various enzymes was still significantly higher than the control group, but the content of malondialdehyde and H_2O_2 increased sharply.