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目的探讨血管内皮祖细胞(EPC)局部移植防治血管成形术后再狭窄形成的可行性。方法分离、鉴定并培养新西兰大白兔外周血(EPC),用2．5F球囊扩张并损伤兔右侧颈动脉血管内皮,对损伤血管进行局部EPC移植。共作细胞移植兔13只,其中3只移植荧光标记EPC;对照组8只,局部灌注生理盐水。细胞移植后4 d,对2只荧光标记细胞移植兔取移植细胞后受损伤血管行病理组织学检查,其余实验兔4周后对损伤血管行病理组织学检查。结果荧光标记细胞移植后4d,病理学检测显示损伤血管内皮有强荧光表达;4周后,细胞移植组血管壁轻度增厚,对照组血管壁增厚明显,血管腔明显狭窄。两组间血管壁厚度差异有统计学意义(P＜0．01)。结论介入法局部移植同种异体血管内皮祖细胞可防治血管成形术后再狭窄的形成。
Objective To investigate the feasibility of local transplantation of vascular endothelial progenitor cells (EPC) for prevention of restenosis after angioplasty. Methods New Zealand white rabbits were isolated, identified, and cultured. EPCs were implanted into damaged blood vessels by 2.5F balloon dilatation and injury of the right carotid artery in rabbits. A total of 13 rabbits were transplanted with cells, of which 3 were transplanted with fluorescent-labeled EPCs and 8 in the control group. Four days after cell transplantation, the damaged blood vessels were implanted into 2 fluorescently labeled cells and histopathological examination was performed on the damaged blood vessels. The other 4 weeks after the transplantation, histopathological examination was performed on the damaged blood vessels. Results Four days after transplantation of fluorescent labeled cells, the pathological examination showed that there was a strong fluorescence expression in the injured vascular endothelium. After 4 weeks, the vessel wall of the cell transplantation group was slightly thickened, while the vascular wall of the control group was thickened obviously. The difference of vascular wall thickness between the two groups was statistically significant (P <0.01). Conclusion Local interventional transplantation of allogenic endothelial progenitor cells can prevent the formation of restenosis after angioplasty.